CRTC3 Antibody (YA1748)

Cat. No.: HY-P82003: Data Sheet COA
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User Guide for Antibodies
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CRTC3 Antibody (YA1748) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRTC3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CRTC3 Antibody (YA1748) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CRTC3 Antibody (YA1748) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRTC3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 67 kDa;

Observed band size: 78 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q6UUV7

Gene ID

64784 [NCBI]

Immunogen

A synthetic peptide of human CRTC3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), SiHa (lane 2, 15 μg), SK-OV-3 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), PC-12 (lane 5, 15 μg), RAW264.7 (lane 6, 15 μg) using CRTC3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is primarily expressed in B cells and T cells. The highest expression level is found in lung tissue. It is also expressed in the brain, colon, heart, kidneys, ovaries, and prostate. Expression is weaker in the liver, pancreas, muscles, small intestine, spleen, and stomach.

Isoforms & Post-Translational Modification：Q6UUV7 has 2 isomers: Q6UUV7-1: 66959 Da (predicted); Q6UUV7-3: 66830 Da (predicted).
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5 (PubMed:30611118). Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus (PubMed:30611118)

Subunit：Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter (PubMed:14506290). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:14506290). Interacts (when phosphorylated at Ser-162 and Se-273) with 14-3-3 proteins (PubMed:30611118). Interacts with YWHAE (PubMed:30611118). Interacts (when phosphorylated at Ser-391) with phosphatase PP2A catalytic subunit PPP2CA and regulatory subunits PPP2R1A and PPP2R2A (PubMed:30611118). Interacts, via the N-terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription (PubMed:17644518)

Database

Entrez Gene: 64784 Human ; 70461 Mouse ; 365297 Rat

SwissProt: Q6UUV7 Human ; Q91X84 Mouse ;

OMIM: 608986 Human

Research Field

Microbiology

Synonyms

TORC3; FLJ21868; CRTC3

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CRTC3 Antibody (YA1748) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CRTC3 Antibody (YA1748)

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Background

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CRTC3 Antibody (YA1748) Related Classifications

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