General Transcription Factor II I Antibody (YA3268)(PBS only)

Cat. No.: HY-P83523A: Data Sheet User Guide for Antibodies
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General Transcription Factor II I Antibody (YA3268) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to General Transcription Factor II I.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

General Transcription Factor II I Antibody (YA3268) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to General Transcription Factor II I.

Host

Rabbit

Molecular Weight

Predicted band size: 112 kDa;

Observed band size: 135,138 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P78347

Gene ID

2969 [NCBI]

Immunogen

A synthetic peptide of human TFII I aa66-80.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in human brain, muscle, and lymphoblasts and is almost undetectable in other human tissues, while the other isoforms are equally expressed in all human tissues

Isoforms & Post-Translational Modification：P78347 has 5 isomers: P78347-1: 112416 Da (predicted); P78347-2: 107970 Da (predicted); P78347-3: 110280 Da (predicted); P78347-4: 110106 Da (predicted); P78347-5: 30409 Da (predicted).
Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation;Sumoylated

Subunit：Homodimer (Potential). Interacts with SRF and PHOX1. Binds a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1). Associates with the PH domain of Bruton's tyrosine kinase (BTK). May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with BTK and ARID3A. Interacts with isoform beta of PRKG1. Interacts with GPR50 (C-TERMINAL DOMAIN)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

GTF2I; BAP135; WBSCR6; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region

Documentation

User Guide for Antibodies (1077 KB)