NOTCH4 Antibody (YA6682)

Cat. No.: HY-P86989: Data Sheet User Guide for Antibodies
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NOTCH4 Antibody (YA6682) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOTCH4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NOTCH4 Antibody (YA6682) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOTCH4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 210 kDa;

Observed band size: 250 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

Q99466

Gene ID

4855 [NCBI]

Immunogen

Synthetic peptide within Human NOTCH4 aa 1,920-1,960 / 2,003.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity)

Subcellular Localization：Cell membrane,Nucleus

Expression：
Tissue_Specificity: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes

Isoforms & Post-Translational Modification：Q99466 has three isomers: Q99466-1: 209622 Da (predicted); Q99466-2: 61262 Da (predicted); Q99466-3: 39617 Da (predicted).
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form丨Phosphorylated

Subunit：Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity)

Synonyms

FLJ16302 antibody; hNotch 4 antibody; hNotch4 antibody; INT3 antibody; Neurogenic locus notch homolog protein 4 antibody; NOTC4_HUMAN antibody; Notch 4 antibody; Notch 4 intracellular domain antibody; Notch homolog 4 antibody; NOTCH3 antibody; FLJ16302 antibody; hNotch 4 antibody; hNotch4 antibody; INT3 antibody; Neurogenic locus notch homolog protein 4 antibody; NOTC4_HUMAN antibody; Notch 4 antibody; Notch 4 intracellular domain antibody; Notch homolog 4 antibody; NOTCH3 antibody; Notch4 antibody; NOTCH4 protein antibody;

Documentation

User Guide for Antibodies (1077 KB)