GAPDH Antibody (YA848)

Cat. No.: HY-P80954: Data Sheet COA
SDS
User Guide for Antibodies
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GAPDH Antibody (YA848) is a non-conjugated and Mouse origined IgM monoclonal antibody. It can be used as a loading control antibody.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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GAPDH Antibody (YA848) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

GAPDH Antibody (YA848) is a non-conjugated and Mouse origined IgM monoclonal antibody. It can be used as a loading control antibody.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey, Rat, Mouse, Saccharomyces cerevisiae, Pichia pastoris, Hamster

SwissProt ID

P04406

Gene ID

2597 [NCBI]

Immunogen

Synthetic peptide corresponding to Human GAPDH aa72-248.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgM

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293(lane 2(20μg), Hela(lane 3(20μg), K562(lane 4(20μg) ) ,RAW264.7(lane 5(20μg) ) using Hsp90 beta (HY-P80187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80187, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), C6 (lane 4, 15 μg), Mouse liver (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using GAPDH Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)

Subcellular Localization：Cytoplasm, cytosol; Nucleus; Cytoplasm, perinuclear region; Membrane; Cytoplasm, cytoskeleton

Subunit：Homotetramer (PubMed:16239728, PubMed:16510976).

RRID

AB_3102771

Database

Entrez Gene: 2597 Human ; 100042025 Mouse ; 14433 Mouse ; 24383 Rat 685186

SwissProt: P04406 Human ; P16858 Mouse ; P04797 Rat

OMIM: 138400 Human

Synonyms

G3PD; GAPD; HEL-S-162eP

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

GAPDH Antibody (YA848) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GAPDH Antibody (YA848)

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Background

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GAPDH Antibody (YA848) Related Classifications

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