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Prostaglandin E Synthase Antibody (YA1741)(PBS only)

(Synonyms: PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1)

Prostaglandin E Synthase Antibody (YA1741) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostaglandin E Synthase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Prostaglandin E Synthase Antibody (YA1741) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostaglandin E Synthase.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13-17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Immunogen

A synthetic peptide of human Prostaglandin E Synthase aa42-59.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
    Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Prostaglandin E Synthase Antibody (HY-P81996) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
    Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Prostaglandin E Synthase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Prostaglandin E Synthase is a Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli. Plays a key role in inflammation response, fever and pain. Also catalyzes the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2. In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively[1][2][3][4][5][6].

  • Subcellular Localization

    Membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region

  • Expression


    Induction:Induced by the interleukin IL1B (PubMed:10377395, PubMed:10760517) . Induced By p53/TP53 (PubMed:9305847)

  • Subunit

    Homotrimer

  • SwissProt ID

    O14684

  • Gene ID
    9536 [NCBI]
  • Synonyms

    PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1

  • Research Field

    Immunology

Prostaglandin E Synthase Antibody (YA1741)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Prostaglandin E Synthase Antibody (HY-P81996) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Prostaglandin E Synthase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Prostaglandin E Synthase Antibody (HY-P81996) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Prostaglandin E Synthase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Experimental Validation Results for Prostaglandin E Synthase Antibody (YA1741)(PBS only)
  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Prostaglandin E Synthase Antibody (HY-P81996) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 3T3 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), Hela (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using Prostaglandin E Synthase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image WB
  • Prostaglandin E Synthase Antibody (YA1741)(PBS only) Product Image WB