PTF1A Antibody (YA1707)(PBS only)

Cat. No.: HY-P81962A: Data Sheet User Guide for Antibodies
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PTF1A Antibody (YA1707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PTF1A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PTF1A Antibody (YA1707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PTF1A.

Host

Rabbit

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q9QX98

Gene ID

19213 [NCBI]

Immunogen

Recombinant protein of mouse PTF1A

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor implicated in the cell fate determination in various organs (PubMed:11562365, PubMed:12185368, PubMed:15543146, PubMed:17075007, PubMed:9851981). Binds to the E-box consensus sequence 5'-CANNTG-3' (PubMed:11562365, PubMed:12185368, PubMed:9851981). Plays a role in early and late pancreas development and differentiation (PubMed:11562365, PubMed:21852532). Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates (PubMed:11562365, PubMed:12185368, PubMed:9851981). May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase (PubMed:11562365, PubMed:12185368, PubMed:9851981). Required for the formation of pancreatic acinar and ductal cells (PubMed:11562365). Plays an important role in cerebellar development (PubMed:15543146). Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates (PubMed:17075007)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Expressed in precursors of pancreatic islets, acini and ducts

Subunit：Component of the pancreas transcription factor 1 complex (PTF1) which is composed of TCF3/p75, TCF12/p64 and PTF1A/p48. TCF3 is responsible for the nuclear import of the p48/p64 complex. Interacts with TCF3 and RBPSUH/RBP-Jkappa

Research Field

Neuroscience

Synonyms

Pancreas-specific transcription factor 1a; bHLH transcription factor p48; PTF1-p48

Documentation

User Guide for Antibodies (1077 KB)