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MAP2K6 Antibody (YA1088)(PBS only)

(Synonyms: MAP2K6; MEK6; MKK6; PRKMK6; SKK3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPKK 6; MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; SAPKK-3; SAPKK3)

MAP2K6 Antibody (YA1088) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP2K6.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MAP2K6 Antibody (YA1088)(PBS only)
  • MAP2K6 Antibody (YA1088)(PBS only) Product Image
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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

MAP2K6 Antibody (YA1088) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP2K6.

  • Host Mouse
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Immunogen

Purified recombinant human MKK6 protein fragments expressed in E.coli.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAP2K6 Antibody (YA1088)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using MAP2K6 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    MAP2K6 dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Isomer 2 is expressed only in skeletal muscle. Isomer 1 is expressed in skeletal muscle and heart, and less frequently in liver or pancreas.

    Induction:Strongly activated by UV, anisomycin, and osmotic shock but not by phorbol esters, NGF or EGF

  • Isoforms & Post-Translational Modification

    P52564 has 2 isomers: P52564-1: 37492 Da (predicted); P52564-2: 31339 Da (predicted).
    Weakly autophosphorylated (PubMed:16728640, PubMed:24936062). Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (PubMed:16728640, PubMed:24936062);In response to genotoxic stress, MAP3K-phosphorylated MAP2K6 is ubiquitinated and degraded by the SCF(FBXO31) complex;(Microbial infection) Acetylation of Ser-207 and Thr-211 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway

  • Subunit

    Dimer. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity).

  • SwissProt ID

    P52564

  • Gene ID
    5608 [NCBI]
  • Synonyms

    MAP2K6; MEK6; MKK6; PRKMK6; SKK3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPKK 6; MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; SAPKK-3; SAPKK3

  • Research Field

    Signal Transduction

MAP2K6 Antibody (YA1088)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using MAP2K6 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MAP2K6 Antibody (YA1088)(PBS only) Product Image
    1/16
  • Experimental Validation Results for MAP2K6 Antibody (YA1088)(PBS only)
  • MAP2K6 Antibody (YA1088)(PBS only) Product Image
  • Experimental Validation Results for MAP2K6 Antibody (YA1088)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using MAP2K6 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MAP2K6 Antibody (YA1088)(PBS only)
  • MAP2K6 Antibody (YA1088)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using MAP2K6 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MAP2K6 Antibody (YA1088)(PBS only) Product Image WB