Copy Link Print Information

PFKFB3 Antibody (YA7475)

Customer Review

Based on 1 Customer Validation

PFKFB3 Antibody (YA7475) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to PFKFB3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PFKFB3 Antibody (YA7475)
  • PFKFB3 Antibody (YA7475) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

PFKFB3 Antibody (YA7475) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to PFKFB3.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PFKFB3 Antibody (YA7475)
    Western blot analysis of extracts from A549 (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Rat spleen (lane 4(20μg)) and Rat thymus (lane 5(20μg)) using PFKFB3 Antibody (HY-P87790) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PFKFB3 catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate

  • Expression


    Tissue_Specificity: Ubiquitous

  • Isoforms & Post-Translational Modification

    Q16875 has four isomers: Q16875-1: 59609 Da (predicted); Q16875-2: 58876 Da (predicted); Q16875-3: 57287 Da (predicted); Q16875-4: 60602 Da (predicted).
    Phosphorylation by AMPK stimulates activity

  • Subunit

    Homodimer (PubMed:16316985)

  • SwissProt ID

    Q16875

PFKFB3 Antibody (YA7475) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Rat spleen (lane 4(20μg)) and Rat thymus (lane 5(20μg)) using PFKFB3 Antibody (HY-P87790) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PFKFB3 Antibody (YA7475) Product Image
    1/16
  • Experimental Validation Results for PFKFB3 Antibody (YA7475)
  • PFKFB3 Antibody (YA7475) Product Image
  • Experimental Validation Results for PFKFB3 Antibody (YA7475)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Rat spleen (lane 4(20μg)) and Rat thymus (lane 5(20μg)) using PFKFB3 Antibody (HY-P87790) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PFKFB3 Antibody (YA7475)
  • PFKFB3 Antibody (YA7475) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Rat spleen (lane 4(20μg)) and Rat thymus (lane 5(20μg)) using PFKFB3 Antibody (HY-P87790) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PFKFB3 Antibody (YA7475) Product Image WB