ABCB4 Antibody
(Synonyms: MDR3, PGY3, ABCB4, Phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, Multidrug resistance protein 3, P-glycoprotein 3)ABCB4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MDR3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
ABCB4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MDR3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 144 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 135; 140; 141 kDa
Purified recombinant protein of human MDR3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MDR3 is an Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts. Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes. Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner. Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane. In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts. Does not confer multidrug resistance (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cell membrane; Apical cell membrane; Membrane raft; Cytoplasm; Cytoplasmic vesicle, clathrin-coated vesicle
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Expression
Induction: Up-regulated by PPARA. Up-regulated by compounds that cause peroxisome proliferation, such as fenofibrate (at protein level). Up-regulated by bezafibrate. Up-regulated by compounds that cause peroxisome proliferation, such as fenofibrate, bezafibrate and gemfibrozil. -
Isoforms & Post-Translational Modification
MDR3 has 3 isoforms, P21439-1: amino acid length is 1286, molecular weight is 141523 Da (predicted); P21439-2: amino acid length is 1279, molecular weight is 140682 Da (predicted); P21439-3: amino acid length is 1232, molecular weight is 135259 Da (predicted).可发生磷酸化修饰(PubMed:24723470)。第 34 位苏氨酸(Thr-34)的磷酸化是 PC 外流活性所必需的。磷酸化发生在丝氨酸和苏氨酸残基上，且依赖蛋白激酶 A 或蛋白激酶 C(PubMed:24723470)。第 44 位苏氨酸(Thr-44)和第 49 位丝氨酸(Ser-49)也可能发生磷酸化(PubMed:24723470)MDR3 存在 3 个异构体，P21439-1：氨基酸个数为 1286 个，分子量为 141523 Da (预测)；P21439-2：氨基酸个数为 1279 个，分子量为 140682 Da (预测)；P21439-3：氨基酸个数为 1232 个，分子量为 135259 Da (预测)。Phosphorylated (PubMed:24723470). Phosphorylation on Thr-34 is required for PC efflux activity. Phosphorylation occurs on serine and threonine residues in a protein kinase A- or C-dependent manner (PubMed:24723470). May be phosphorylated on Thr-44 and Ser-49 (PubMed:24723470)
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Subunit
May interact with RACK1.
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SwissProt ID
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Synonyms
MDR3, PGY3, ABCB4, Phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, Multidrug resistance protein 3, P-glycoprotein 3
Documentation
[1]. Morita SY, et al. Bile salt-dependent efflux of cellular phospholipids mediated by ATP binding cassette protein B4. Hepatology. 2007 Jul;46(1):188-99. [Content Brief]
[2]. Groen A, et al. Complementary functions of the flippase ATP8B1 and the floppase ABCB4 in maintaining canalicular membrane integrity. Gastroenterology. 2011 Nov;141(5):1927-37.e1-4. [Content Brief]
[3]. Morita SY, et al. Bile salt-stimulated phospholipid efflux mediated by ABCB4 localized in nonraft membranes. J Lipid Res. 2013 May;54(5):1221-30. [Content Brief]
[4]. Gordo-Gilart R, et al. Functional analysis of ABCB4 mutations relates clinical outcomes of progressive familial intrahepatic cholestasis type 3 to the degree of MDR3 floppase activity. Gut. 2015 Jan;64(1):147-55. [Content Brief]
[5]. Gautherot J, et al. Phosphorylation of ABCB4 impacts its function: insights from disease-causing mutations. Hepatology. 2014 Aug;60(2):610-21. [Content Brief]
[6]. Andress EJ, et al. Molecular mechanistic explanation for the spectrum of cholestatic disease caused by the S320F variant of ABCB4. Hepatology. 2014 May;59(5):1921-31. [Content Brief]
[7]. Olsen JA, et al. Structure of the human lipid exporter ABCB4 in a lipid environment. Nat Struct Mol Biol. 2020 Jan;27(1):62-70. [Content Brief]
[8]. Smith AJ, et al. The human MDR3 P-glycoprotein promotes translocation of phosphatidylcholine through the plasma membrane of fibroblasts from transgenic mice. FEBS Lett. 1994 Nov 14;354(3):263-6. [Content Brief]
[9]. van Helvoort A, et al. MDR1 P-glycoprotein is a lipid translocase of broad specificity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine. Cell. 1996 Nov 1;87(3):507-17. [Content Brief]
[10]. Crawford AR, et al. Hepatic secretion of phospholipid vesicles in the mouse critically depends on mdr2 or MDR3 P-glycoprotein expression. Visualization by electron microscopy. J Clin Invest. 1997 Nov 15;100(10):2562-7. [Content Brief]
[11]. Degiorgio D, et al. Two ABCB4 point mutations of strategic NBD-motifs do not prevent protein targeting to the plasma membrane but promote MDR3 dysfunction. Eur J Hum Genet. 2014 May;22(5):633-9. [Content Brief]
[12]. Delaunay JL, et al. Functional defect of variants in the adenosine triphosphate-binding sites of ABCB4 and their rescue by the cystic fibrosis transmembrane conductance regulator potentiator, ivacaftor (VX-770). Hepatology. 2017 Feb;65(2):560-570. [Content Brief]
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