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Semaglutide Antibody

Semaglutide Antibody is Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Semaglutide.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS(pH 7.4), 50% Glycerol, 0.05% Proclin 300.

  • Experimental Validation Results for Semaglutide Antibody
  • Semaglutide Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:50-1:500 1:5000-20000

Product Details

Description

Semaglutide Antibody is Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Semaglutide.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human
Immunogen

Synthesized Semaglutide.

Sensitivity

Transfected

Purification

affinity purified

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS(pH 7.4), 50% Glycerol, 0.05% Proclin 300.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Semaglutide Antibody
    Indirect ELISA analysis of Semaglutide was performed by coating wells of a 96-well plate with 100 µl per well of GLP-1(7-36) antigen (HY-P0054A, Red), GLP-1(7-36) antigen (HY-P1145, Blue), Semaglutide antigen (HY-114118, Black), Tirzepatide antigen (HY-P1731, Green) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Semaglutide antibody (HY-P810528), starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.01 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

Background

  • Function

    Semaglutide is a long-acting, selective, competitive GLP-1R agonist that can penetrate the blood-brain barrier. After activating GLP-1R, Semaglutide promotes insulin secretion, inhibits gastric emptying and appetite, and at the same time enhances autophagy, inhibits oxidative stress and apoptosis. Semaglutide also regulates mitochondrial function and lipid metabolism (such as reducing de novo lipogenesis in the liver). Semaglutide has activities such as lowering blood sugar, reducing weight, neuroprotection (such as improving motor function in Parkinson's disease models, reducing α-synuclein aggregation) and improving hepatic steatosis. Semaglutide can be used for the study of neurodegenerative diseases and liver diseases such as type 2 diabetes, obesity, Parkinson's disease, metabolic associated fatty liver disease (MASLD), and cancer.

Semaglutide Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Indirect ELISA analysis of Semaglutide was performed by coating wells of a 96-well plate with 100 µl per well of GLP-1(7-36) antigen (HY-P0054A, Red), GLP-1(7-36) antigen (HY-P1145, Blue), Semaglutide antigen (HY-114118, Black), Tirzepatide antigen (HY-P1731, Green) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Semaglutide antibody (HY-P810528), starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.01 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • Semaglutide Antibody Product Image
    1/16
  • Experimental Validation Results for Semaglutide Antibody
  • Semaglutide Antibody Product Image
  • Experimental Validation Results for Semaglutide Antibody
    1/1
    MedChemExpress Validation
     Indirect ELISA analysis of Semaglutide was performed by coating wells of a 96-well plate with 100 µl per well of GLP-1(7-36) antigen (HY-P0054A, Red), GLP-1(7-36) antigen (HY-P1145, Blue), Semaglutide antigen (HY-114118, Black), Tirzepatide antigen (HY-P1731, Green) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Semaglutide antibody (HY-P810528), starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.01 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.
  • Experimental Validation Results for Semaglutide Antibody
  • Semaglutide Antibody Product Image
    MedChemExpress Validation

    Indirect ELISA analysis of Semaglutide was performed by coating wells of a 96-well plate with 100 µl per well of GLP-1(7-36) antigen (HY-P0054A, Red), GLP-1(7-36) antigen (HY-P1145, Blue), Semaglutide antigen (HY-114118, Black), Tirzepatide antigen (HY-P1731, Green) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Semaglutide antibody (HY-P810528), starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.01 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • Semaglutide Antibody Product Image ELISA