Islet 1 Antibody (YA1244)

製品番号: HY-P81499: データシート SDS COA User Guide for Antibodies
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Islet 1 Antibody (YA1244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Islet 1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Islet 1 Antibody (YA1244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Islet 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 39 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey, Pig

SwissProt ID

P61371

Gene ID

3670 [NCBI]

Immunogen

Synthetic peptide within Human Islet 1 aa 262-205 / 349.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
FC FC: Flow Cytometry	1:1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500
IP IP: Immunoprecipitation	1-2μg/sample

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL WB IHC-P ICC

Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) , Neuro-2a(lane 3(20ug) and HEK293(lane 4(20ug) using Islet 1 Antibody (HY-P81499) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Islet 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Islet 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of SH-SY5Y cells labeling Islet 1 with Islet 1 Antibody (YA1244) (HY-P81499) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Islet 1 Antibody (YA1244) ((HY-P81499) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of SH-SY5Y cells labeling Islet 1 with Islet 1 Antibody (YA1244) (HY-P81499) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Islet 1 Antibody (YA1244) ((HY-P81499) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:This gene is expressed in some neurons of the adrenal medulla and dorsal root ganglion, the nuclear layer and ganglion cell layer of the retina, the pineal gland, and certain areas of the brain.
Positive sample: SH-SY5Y cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, SH-SY5Y, Neuro-2a, human pancreas tissue, mouse embryo tissue, rat pancreas tissue.

Subunit：At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1; allosteric structural changes in the DNA binding domain of LHX3, induced by the ISL1:LHX3 interaction, may explain differences in sequence specificity of the different complexes. Interacts with LHX3. Interacts (via C-terminus) with POU4F2 (via C-terminus) isoform 1. Interacts with POU3F2. Interacts with POU4F3. Interacts (via N-terminal domain) with MLIP; the interaction represses ISL1 transactivator activity. Interacts with GCN5/KAT2A. Interactions of ISL1 with MLIP1 or KAT2A may be mutually exclusive (By similarity)

RRID

AB_3103613

Database

Entrez Gene: 3670 Human ; 16392 Mouse ; 64444 Rat

SwissProt: P61371 Human ; P61372 Mouse ; P61374 Rat

OMIM: 600366 Human

Research Field

Neuroscience

Synonyms

Insulin gene enhancer protein ISL 1 antibody; Insulin gene enhancer protein ISL-1 antibody; Insulin related protein antibody; ISL 1 antibody; ISL LIM homeobox 1 antibody; ISL1 antibody; ISL1 transcription factor LIM homeodomain antibody; ISL1 transcription factor, LIM/homeodomain (islet 1) antibody; ISL1 transcription factor, LIM/homeodomain antibody; ISL1_HUMAN antibody; Islet-1 antibody; Islet1 antibody;

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)