BRD2 Antibody (YA3910)(PBS only)

Cat. No.: HY-P84213A: Data Sheet User Guide for Antibodies
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BRD2 Antibody (YA3910) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to BRD2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BRD2 Antibody (YA3910) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to BRD2.

Host

Mouse

Molecular Weight

Predicted band size: 88 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P25440

Gene ID

6046 [NCBI]

Immunogen

Purified recombinant fragment of human BRD2 (AA: 227-364) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed:17148447, PubMed:17848202, PubMed:18406326, PubMed:20048151, PubMed:20709061, PubMed:20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed:35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed:28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed:28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed:37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed:37731000)

Subcellular Localization：Nucleus; Chromosome

Isoforms & Post-Translational Modification：P25440 has 4 isomers: P25440-1: 88061 Da (predicted); P25440-2: 92033 Da (predicted); P25440-3: 83151 Da (predicted); P25440-4: 74881 Da (predicted).

Subunit：Homodimer (PubMed:17148447, PubMed:17848202, PubMed:20048151, PubMed:20709061). Interacts with E2F1 (PubMed:17148447). Interacts with (acetylated) STAT3; promoting STAT3 recruitment to chromatin (PubMed:28262505). Interacts with CTCF; promoting BRD2 recruitment to chromatin (By similarity)

Synonyms

FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1

Documentation

User Guide for Antibodies (1077 KB)