CD63 Antibody (YA530)(PBS only)

Cat. No.: HY-P80604A: Data Sheet User Guide for Antibodies
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CD63 Antibody (YA530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

CD63 Antibody (YA530) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.

Host

Rabbit

Molecular Weight

Predicted band size: 26 kDa;

Observed band size: 40-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

P41731

Gene ID

967 [NCBI]

Immunogen

Synthetic peptide corresponding to Mouse CD63.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:20000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:400
IF	1:50

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01 M PBS, pH 7.2.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAW264.7(lane 2(20μg) and RAW264.7(lane 3(40μg) using CD63 Antibody (HY-P80604) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Endosome, multivesicular body; Melanosome; Secreted, extracellular exosome; Cell surface

Expression：
Tissue_specificity:Ubiquitous. Strongly expressed in kidney. Detected in spleen, bone marrow, peripheral blood mononuclear cells and macrophages

Subunit：Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL.

Research Field

Cardiovascular

Synonyms

CD63 antigen; CD63

Documentation

User Guide for Antibodies (1077 KB)