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PRMT7 Antibody (YA1734)

(Synonyms: SBIDDS)
Customer Review

Based on 1 Customer Validation

PRMT7 Antibody (YA1734) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • PRMT7 Antibody (YA1734) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PRMT7 Antibody (YA1734) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT7.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PRMT7

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104110

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRMT7 Antibody (YA1734)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 293 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using PRMT7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
    Western blot analysis of extracts from K562(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT7 Antibody (HY-P81989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PRMT7 is a unique member of the protein arginine methyltransferase (PRMT) family that catalyzes the ω-monomethylation of arginine residues in a substrate-specific manner[1][2][3]. Mechanistically, PRMT7 preferentially targets arginine residues within R-X-R motifs flanked by basic amino acids and exhibits optimal enzymatic activity at lower-than-physiological temperatures, distinguishing it from other PRMT isoforms[1][4]. PRMT7-mediated arginine methylation regulates chromatin structure, RNA splicing, stress response, and cell stemness, thereby influencing gene expression, muscle stem cell physiology, B cell immunity, and neural development[2][3][5][6]. In disease models, PRMT7 deficiency in mice causes defects in muscle satellite cells and immune cell function, while humans with PRMT7 mutations display intellectual disability, hypotonia, and facial dysmorphisms, highlighting its developmental relevance[2][3]. Compared with PRMT5 and PRMT9, PRMT7 exclusively produces monomethylarginine, lacks significant symmetric or asymmetric dimethylation, and shows distinct substrate recognition, particularly for histone H2B and R-X-R motifs[1][4][7]. For experimental applications, selective PRMT7 inhibitors have been developed, enabling modulation of its methyltransferase activity in cellular assays and cancer models, with co-inhibition of PRMT4/5/7 demonstrating synergistic suppression of tumor cell growth[2][6][8]. Collectively, PRMT7 functions as a context-dependent regulator of arginine methylation with implications for epigenetic regulation, RNA processing, and disease modeling[1][2][3][4][6].

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus

  • Isoforms & Post-Translational Modification

    Q9NVM4 has 4 isomers: Q9NVM4-1: 78459 Da (predicted); Q9NVM4-2: 62118 Da (predicted); Q9NVM4-3: 73154 Da (predicted); Q9NVM4-4: 63806 Da (predicted).

  • Subunit

    Homodimer and heterodimer (By similarity). Interacts with CTCFL (By similarity). Interacts with PRMT5 and SNRPD3

  • SwissProt ID

    Q9NVM4

  • Gene ID
    54496 [NCBI]
  • Synonyms

    SBIDDS

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Miranda TB, et al. PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity. J Biol Chem. 2004 May 28;279(22):22902-7. [Content Brief]

[2]. Jain K, et al. PRMT7 as a unique member of the protein arginine methyltransferase family: A review. Arch Biochem Biophys. 2019 Apr 15;665:36-45. [Content Brief]

[3]. Hsieh HC, et al. PRMT-7/PRMT7 activates HLH-30/TFEB to guard plasma membrane integrity compromised by bacterial pore-forming toxins. Autophagy. 2024 Jun;20(6):1335-1358. [Content Brief]

[4]. Zurita-Lopez CI, et al. Human protein arginine methyltransferase 7 (PRMT7) is a type III enzyme forming ω-NG-monomethylated arginine residues. J Biol Chem. 2012 Mar 9;287(11):7859-70. [Content Brief]

[5]. Wang CD, et al. Developmental expression of three prmt genes in Xenopus. Zool Res. 2019 Mar 18;40(2):102-107. [Content Brief]

[6]. Li WJ, et al. Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth. Nat Commun. 2021 Mar 29;12(1):1946. [Content Brief]

[7]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]

[8]. Feoli A, et al. Identification of a Protein Arginine Methyltransferase 7 (PRMT7)/Protein Arginine Methyltransferase 9 (PRMT9) Inhibitor. J Med Chem. 2023 Oct 12;66(19):13665-13683. [Content Brief]

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PRMT7 Antibody (YA1734) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 293 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using PRMT7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT7 Antibody (HY-P81989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT7 Antibody (YA1734) Product Image
    1/16
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • PRMT7 Antibody (YA1734) Product Image
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 293 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using PRMT7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT7 Antibody (HY-P81989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • Experimental Validation Results for PRMT7 Antibody (YA1734)
  • PRMT7 Antibody (YA1734) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 293 (lane 3, 15 μg), MCF-7 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using PRMT7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PRMT7 Antibody (YA1734) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT7 Antibody (HY-P81989) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT7 Antibody (YA1734) Product Image WB
  • PRMT7 Antibody (YA1734) Product Image WB