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G-CSF Receptor Antibody (YA4441)

(Synonyms: CSF3R; SCN7; GCSFR)
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Based on 1 Customer Validation

G-CSF Receptor Antibody (YA4441) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to G-CSF Receptor.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for G-CSF Receptor Antibody (YA4441)
  • G-CSF Receptor Antibody (YA4441) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

G-CSF Receptor Antibody (YA4441) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to G-CSF Receptor.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 92 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 92 kDa
Immunogen

Purified recombinant fragment of human CD114 (AA: extra 25-187) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for G-CSF Receptor Antibody (YA4441)
    Flow cytometric analysis of 1X10^6 Ramos cells labeling CD114 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    G-CSF Receptor receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface

  • Subcellular Localization

    Secreted; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:One or more isoforms were found in the myeloid leukemia cell line KG-1, the leukemia U-937 cell line, bone marrow cells, placenta, and peripheral blood granulocytes. The isoform GCSFR-2 was present only in leukemia U-937 cells. The isoform GCSFR-3 was highly expressed in the placenta.

  • Isoforms & Post-Translational Modification

    Q99062 has 4 isomers: Q99062-1: 92156 Da (predicted); Q99062-2: 85120 Da (predicted); Q99062-3: 95114 Da (predicted); Q99062-4: 86609 Da (predicted).
    N-glycosylated

  • Subunit

    Homodimer. The dimeric receptor binds two CSF3 molecules.

  • SwissProt ID

    Q99062

  • Gene ID
    1441 [NCBI]
  • Synonyms

    CSF3R; SCN7; GCSFR

G-CSF Receptor Antibody (YA4441) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling CD114 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • G-CSF Receptor Antibody (YA4441) Product Image
    1/16
  • Experimental Validation Results for G-CSF Receptor Antibody (YA4441)
  • G-CSF Receptor Antibody (YA4441) Product Image
  • Experimental Validation Results for G-CSF Receptor Antibody (YA4441)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling CD114 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for G-CSF Receptor Antibody (YA4441)
  • G-CSF Receptor Antibody (YA4441) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Ramos cells labeling CD114 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • G-CSF Receptor Antibody (YA4441) Product Image FC