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RRM2 Antibody (YA1885)(PBS only)

(Synonyms: R2; RR2M)

RRM2 Antibody (YA1885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RRM2 Antibody (YA1885)(PBS only)
  • RRM2 Antibody (YA1885)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

RRM2 Antibody (YA1885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM2.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

Recombinant protein of human RRM2

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RRM2 Antibody (YA1885)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), K562 (lane 3, 20 μg), Jurkat (lane 4, 20 μg), A431 (lane 5, 20 μg), and HUVEC (lane 6, 20 μg) using RRM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    RRM2 is a Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Induction:Up-regulated in response to DNA damage induced by doxorubicin, camptothecin, UV-C, methyl methanesulfonate, nocodazole, or gamma-irradiation

  • Isoforms & Post-Translational Modification

    P31350 has 2 isomers: P31350-1: 44878 Da (predicted); P31350-2: 51093 Da (predicted).
    Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. Phosphorylated on Thr-33 by CDK1 and CDK2; predominantly in G2 and M phase (PubMed:22632967);Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex; leading to its degradation by the proteasome

  • Subunit

    Heterodimer of a large and a small subunit. Interacts (via Cy motif and when phosphorylated at Thr-33) with CCNF; the interaction occurs exclusively in G2 and early M (PubMed:22632967)

  • SwissProt ID

    P31350

  • Gene ID
    6241 [NCBI]
  • Synonyms

    R2; RR2M

  • Research Field

    Epigenetics and Nuclear Signaling

RRM2 Antibody (YA1885)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), K562 (lane 3, 20 μg), Jurkat (lane 4, 20 μg), A431 (lane 5, 20 μg), and HUVEC (lane 6, 20 μg) using RRM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RRM2 Antibody (YA1885)(PBS only) Product Image
    1/16
  • Experimental Validation Results for RRM2 Antibody (YA1885)(PBS only)
  • RRM2 Antibody (YA1885)(PBS only) Product Image
  • Experimental Validation Results for RRM2 Antibody (YA1885)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), K562 (lane 3, 20 μg), Jurkat (lane 4, 20 μg), A431 (lane 5, 20 μg), and HUVEC (lane 6, 20 μg) using RRM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RRM2 Antibody (YA1885)(PBS only)
  • RRM2 Antibody (YA1885)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), K562 (lane 3, 20 μg), Jurkat (lane 4, 20 μg), A431 (lane 5, 20 μg), and HUVEC (lane 6, 20 μg) using RRM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RRM2 Antibody (YA1885)(PBS only) Product Image WB