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ESE1 Antibody (YA2784)

(Synonyms: Elf3; EPR1; ERT; ESE-1; ESX; jen)
Customer Review

Based on 1 Customer Validation

ESE1 Antibody (YA2784) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ESE1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • ESE1 Antibody (YA2784) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

ESE1 Antibody (YA2784) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ESE1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ESE1 aa1-371.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105155

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ESE1 Antibody (YA2784)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using ESE1 Antibody (YA2784). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ESE1 Antibody (YA2784)
    Flow cytometric analysis of 1X106 NIH3T3 cells labeling ESE1 Antibody(HY-P83039, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ESE1 transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin

    Induction:Transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells. Up-regulated by phorbol 12-myristate 13-acetate (PMA) in bronchial epithelial cells. By retinoic acid in MCF-7 mammary epithelial cells (at protein level)

  • Isoforms & Post-Translational Modification

    P78545 has 2 isomers: P78545-1: 41454 Da (predicted); P78545-2: 39357 Da (predicted).

  • Subunit

    Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function.

  • SwissProt ID

    P78545

  • Gene ID
    1999 [NCBI]
  • Synonyms

    Elf3; EPR1; ERT; ESE-1; ESX; jen

  • Research Field

    Epigenetics and Nuclear Signaling

ESE1 Antibody (YA2784) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using ESE1 Antibody (YA2784). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH3T3 cells labeling ESE1 Antibody(HY-P83039, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ESE1 Antibody (YA2784) Product Image
    1/16
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • ESE1 Antibody (YA2784) Product Image
  • Experimental Validation Results for ESE1 Antibody (YA2784)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using ESE1 Antibody (YA2784). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ESE1 Antibody (YA2784)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH3T3 cells labeling ESE1 Antibody(HY-P83039, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • Experimental Validation Results for ESE1 Antibody (YA2784)
  • ESE1 Antibody (YA2784) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using ESE1 Antibody (YA2784). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ESE1 Antibody (YA2784) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 NIH3T3 cells labeling ESE1 Antibody(HY-P83039, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ESE1 Antibody (YA2784) Product Image WB
  • ESE1 Antibody (YA2784) Product Image FC