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E7 Antibody (YA4820)

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E7 Antibody (YA4820) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to E7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for E7 Antibody (YA4820)
  • E7 Antibody (YA4820) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000 1:50-200

Product Details

Description

E7 Antibody (YA4820) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to E7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
Immunogen

Purified recombinant fragment of E7 expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

RRID

AB_3719018

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for E7 Antibody (YA4820)
    Western blot analysis of extracts from HeLa(lane 2(20μg)), SiHa(lane 3(20μg)), 293T(lane 4(20μg)) and A431(lane 5(20μg)) using E7 Antibody（HY-P85128）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    E7 plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta)

  • Subcellular Localization

    Host cytoplasm; Host nucleus

  • Subunit

    Homodimer (PubMed:11123931). Homooligomer (PubMed:15035602). Interacts with host RB1; this interaction induces dissociation of RB1-E2F1 complex thereby disrupting RB1 activity (PubMed:1316611). Interacts with host EP300; this interaction represses EP300 transcriptional activity (PubMed:12970734). Forms a complex with CHD4 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD4 and HDAC2 may also form (PubMed:10228159). Interacts with protein E2; this interaction inhibits E7 oncogenic activity (PubMed:16439535)

  • SwissProt ID

    P03129

  • Gene ID
    1489079 [NCBI]

E7 Antibody (YA4820) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)), SiHa(lane 3(20μg)), 293T(lane 4(20μg)) and A431(lane 5(20μg)) using E7 Antibody（HY-P85128）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • E7 Antibody (YA4820) Product Image
    1/16
  • Experimental Validation Results for E7 Antibody (YA4820)
  • E7 Antibody (YA4820) Product Image
  • Experimental Validation Results for E7 Antibody (YA4820)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)), SiHa(lane 3(20μg)), 293T(lane 4(20μg)) and A431(lane 5(20μg)) using E7 Antibody（HY-P85128）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for E7 Antibody (YA4820)
  • E7 Antibody (YA4820) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg)), SiHa(lane 3(20μg)), 293T(lane 4(20μg)) and A431(lane 5(20μg)) using E7 Antibody（HY-P85128）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • E7 Antibody (YA4820) Product Image WB