SMG1 Antibody
(Synonyms: 61E3.4, ATX, hSMG-1, hSMG1, KIAA0421, Lambda interacting protein, Lambda-interacting protein, Lambda/iota protein kinase C interacting protein, Lambda/iota protein kinase C-interacting protein, LIP)SMG1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SMG1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:100-500 |
Product Details
SMG1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SMG1.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 410/395/340/269 kDa;
Recombinant protein of human Smg1 (aa 3450-3661.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SMG1 is a Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. -
Isoforms & Post-Translational Modification
SMG1 has 4 isoforms, Q96Q15-1: amino acid length is 3661, molecular weight is 410501 Da (predicted); Q96Q15-2: amino acid length is 3521, molecular weight is 395382 Da (predicted); Q96Q15-3: amino acid length is 3031, molecular weight is 340674 Da (predicted); Q96Q15-4: amino acid length is 2392, molecular weight is 269425 Da (predicted).Autophosphorylated
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Subunit
Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain.
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SwissProt ID
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Synonyms
61E3.4, ATX, hSMG-1, hSMG1, KIAA0421, Lambda interacting protein, Lambda-interacting protein, Lambda/iota protein kinase C interacting protein, Lambda/iota protein kinase C-interacting protein, LIP
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