CNOT7 Antibody
(Synonyms: CAF1, CNOT7, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, Caf1a, CAF-1)CNOT7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CNOT7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
CNOT7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CNOT7.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32 kDa
Synthetic peptide corresponding to the N-term region of human CNOT7.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CNOT7 is a Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex also seems to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Cytoplasm, P-body; Cytoplasm, Cytoplasmic ribonucleoprotein granule
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Isoforms & Post-Translational Modification
CNOT7 has 2 isoforms, Q9UIV1-1: amino acid length is 285, molecular weight is 32745 Da (predicted); Q9UIV1-2: amino acid length is 244, molecular weight is 28228 Da (predicted).CNOT7 存在 2 个异构体，Q9UIV1-1：氨基酸个数为 285 个，分子量为 32745 Da (预测)；Q9UIV1-2：氨基酸个数为 244 个，分子量为 28228 Da (预测)。
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Subunit
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8.
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SwissProt ID
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Synonyms
CAF1, CNOT7, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, Caf1a, CAF-1
Documentation
[1]. Horiuchi M, et al. Structural basis for the antiproliferative activity of the Tob-hCaf1 complex. J Biol Chem. 2009 May 8;284(19):13244-55. [Content Brief]
[2]. Cooke A, et al. Translational repression by deadenylases. J Biol Chem. 2010 Sep 10;285(37):28506-13. [Content Brief]
[3]. Kim TH, et al. Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation. Science. 2019 Aug 23;365(6455):825-829. [Content Brief]
[4]. Aslam A, et al. The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation. Mol Biol Cell. 2009 Sep;20(17):3840-50. [Content Brief]
[5]. Piao X, et al. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells. Mol Cell Biol. 2010 Mar;30(6):1486-94. [Content Brief]
[6]. Doidge R, et al. The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex. PLoS One. 2012;7(12):e51331. [Content Brief]
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