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GNAQ Antibody (YA9799)

(Synonyms: GAQ, SWS, CMAL, CMC1, G-ALPHA-q, GNAQ)
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Based on 1 Customer Validation

GNAQ Antibody (YA9799) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to GNAQ.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GNAQ Antibody (YA9799)
  • GNAQ Antibody (YA9799) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1000 1:200-1000 1:20-50 1:20-50

Product Details

Description

GNAQ Antibody (YA9799) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to GNAQ.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa; Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Immunogen

Recombinant protein of human GNAQ

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GNAQ Antibody (YA9799)
    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2, Negative), Jurkat (lane 3), rat brain (lane 4), rat lung (lane 5), and mouse kidney (lane 6) using GNAQ antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    GNAQ is a Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs). Together with GNA11, required for heart development (By similarity)[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Golgi apparatus; Nucleus; Nucleus membrane

  • Expression


    Tissue_Specificity: Predominantly expressed in ovary, prostate, testis and colon. Down-regulated in the peripheral blood lymphocytes (PBLs) of rheumatoid arthritis patients (at protein level).

  • Isoforms & Post-Translational Modification

    GNAQ has an amino acid length of 359, molecular weight is 42142 Da.
    Palmitoylated by ZDHHC3 and ZDHHC7. Palmitoylation occurs in the Golgi and participates in the localization of GNAQ to the plasma membrane.

  • Subunit

    G proteins are composed of 3 units; alpha, beta and gamma.

  • SwissProt ID

    P50148

  • Synonyms

    GAQ, SWS, CMAL, CMC1, G-ALPHA-q, GNAQ

References

[1]. Zhang X, et al. Structures of the human cholecystokinin receptors bound to agonists and antagonists. Nat Chem Biol. 2021 Dec;17(12):1230-1237. [Content Brief]

[2]. Ding Y, et al. Structural insights into human brain-gut peptide cholecystokinin receptors. Cell Discov. 2022 Jun 7;8(1):55. [Content Brief]

[3]. Falzone ME, et al. The mechanism of Gα(q) regulation of PLCβ3-catalyzed PIP2 hydrolysis. Proc Natl Acad Sci U S A. 2023 Nov 28;120(48):e2315011120. [Content Brief]

[4]. Alvarez-Curto E, et al. Targeted Elimination of G Proteins and Arrestins Defines Their Specific Contributions to Both Intensity and Duration of G Protein-coupled Receptor Signaling. J Biol Chem. 2016 Dec 30;291(53):27147-27159. [Content Brief]

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GNAQ Antibody (YA9799) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2, Negative), Jurkat (lane 3), rat brain (lane 4), rat lung (lane 5), and mouse kidney (lane 6) using GNAQ antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • GNAQ Antibody (YA9799) Product Image
    1/16
  • Experimental Validation Results for GNAQ Antibody (YA9799)
  • GNAQ Antibody (YA9799) Product Image
  • Experimental Validation Results for GNAQ Antibody (YA9799)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2, Negative), Jurkat (lane 3), rat brain (lane 4), rat lung (lane 5), and mouse kidney (lane 6) using GNAQ antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for GNAQ Antibody (YA9799)
  • GNAQ Antibody (YA9799) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2, Negative), Jurkat (lane 3), rat brain (lane 4), rat lung (lane 5), and mouse kidney (lane 6) using GNAQ antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • GNAQ Antibody (YA9799) Product Image WB