IL-5 Antibody
(Synonyms: Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5)IL-5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IL-5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
IL-5 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IL-5.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 16 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 15 kDa
Synthetic peptide corresponding to the center region of human IL-5.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IL-5 is a Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively[1][2][3][4][5].
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Subcellular Localization
Secreted
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Expression
Tissue_Specificity: Present in peripheral blood mononuclear cells. -
Isoforms & Post-Translational Modification
IL-5 has 2 isoforms, P05113-1: amino acid length is 134, molecular weight is 15238 Da (predicted); P05113-2: amino acid length is 123, molecular weight is 13982 Da (predicted).
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Subunit
Homodimer; disulfide-linked.
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SwissProt ID
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Synonyms
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
Documentation
[1]. Clutterbuck EJ, et al. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrow cultures: comparison and interaction with IL-1, IL-3, IL-6, and GMCSF. Blood. 1989 May 1;73(6):1504-12. [Content Brief]
[2]. Ochiai K, et al. IL-5 but not interferon-gamma (IFN-gamma) inhibits eosinophil apoptosis by up-regulation of bcl-2 expression. Clin Exp Immunol. 1997 Jan;107(1):198-204. [Content Brief]
[3]. Tavernier J, et al. Molecular basis of the membrane-anchored and two soluble isoforms of the human interleukin 5 receptor alpha subunit. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7041-5. [Content Brief]
[4]. Kusano S, et al. Structural basis of interleukin-5 dimer recognition by its α receptor. Protein Sci. 2012 Jun;21(6):850-64. [Content Brief]
[5]. Alam R, et al. The interleukin-5/receptor interaction activates Lyn and Jak2 tyrosine kinases and propagates signals via the Ras-Raf-1-MAP kinase and the Jak-STAT pathways in eosinophils. Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):226-7. [Content Brief]
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