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DKK3 Antibody (YA4554)

(Synonyms: RIG; REIC)
Customer Review

Based on 1 Customer Validation

DKK3 Antibody (YA4554) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DKK3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Rabbit, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • DKK3 Antibody (YA4554) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

DKK3 Antibody (YA4554) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DKK3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit, Monkey
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718990

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Western blot analysis of extracts from HEK293(lane 2(20μg)), 293T(lane 3(20μg)), MCF-7(lane 4(20μg)) and HUVEC(lane 5(20μg)) using DKK3 Antibody (HY-P84857). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat heart muscle tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    DKK3 is an Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is expressed at the highest levels in the heart, brain, and spinal cord.

  • Subunit

    Interacts with LRP5 and LRP6

  • SwissProt ID

    Q9UBP4

  • Gene ID
    27122 [NCBI]
  • Synonyms

    RIG; REIC

DKK3 Antibody (YA4554) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg)), 293T(lane 3(20μg)), MCF-7(lane 4(20μg)) and HUVEC(lane 5(20μg)) using DKK3 Antibody (HY-P84857). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart muscle tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    1/16
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • DKK3 Antibody (YA4554) Product Image
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg)), 293T(lane 3(20μg)), MCF-7(lane 4(20μg)) and HUVEC(lane 5(20μg)) using DKK3 Antibody (HY-P84857). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart muscle tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • Experimental Validation Results for DKK3 Antibody (YA4554)
  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 2(20μg)), 293T(lane 3(20μg)), MCF-7(lane 4(20μg)) and HUVEC(lane 5(20μg)) using DKK3 Antibody (HY-P84857). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart muscle tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using DKK3 Antibody (HY-P84857, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DKK3 Antibody (YA4554) Product Image WB
  • DKK3 Antibody (YA4554) Product Image IHC-P
  • DKK3 Antibody (YA4554) Product Image IHC-P
  • DKK3 Antibody (YA4554) Product Image IHC-P
  • DKK3 Antibody (YA4554) Product Image IHC-P
  • DKK3 Antibody (YA4554) Product Image IHC-P
  • DKK3 Antibody (YA4554) Product Image IHC-P