2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. CD11b Antibody (YA3423)

CD11b Antibody (YA3423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.

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사이즈 가격 재고 수량
10 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • 제품 설명

제품 설명

CD11b Antibody (YA3423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.
Host

Rabbit
Clonality

Monoclonal
분자량
Predicted band size: 127 kDa;
Observed band size: 170 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human CD11b aa1101-1152.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
ALL WB FC

  • Western blot analysis of extracts from TF-1(lane 2(20ug) , THP-1(lane 3(20ug) and U937(lane 4(20ug) using CD11b Antibody (HY-P83690) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Flow cytometric analysis of 1X10^6 TF-1 cells labeling CD11b Antibody (YA3423) (HY-P83690, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background
Function：Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity)
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein
Expression：
Tissue_specificity:It is mainly expressed in monocytes and granulocytes (PubMed: 1346576) . It is also expressed in neutrophils (protein level) (PubMed: 21193407) .
Isoforms & Post-Translational Modification：P11215 has 2 isomers: P11215-1: 127179 Da (predicted); P11215-2: 127307 Da (predicted).
Subunit：Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18 (PubMed:21193407). Interacts with JAM3 (PubMed:12208882, PubMed:15194813). Interacts with THBD (PubMed:27055590). Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance (PubMed:20008295, PubMed:9558116). Interacts with TMEM268; this interaction inhibits ITGAM degradation via the endosome-lysosome pathway (PubMed:38730209)
RRID
Database

Entrez Gene: 3684 Human

SwissProt: P11215 Human

OMIM: 609939 Human

Research Field

Neuroscience
Synonyms
CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; CD11b; Leukocyte adhesion receptor MO1
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CD11b Antibody (YA3423) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
CD11b Antibody (YA3423)
Cat. No.:
HY-P83690
수량:
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