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CD11b Antibody (YA3423)

(Synonyms: CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; CD11b; Leukocyte adhesion receptor MO1)
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Based on 1 Customer Validation

CD11b Antibody (YA3423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • CD11b Antibody (YA3423) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50 1:50-1:100

Product Details

Description

CD11b Antibody (YA3423) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11b.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 170 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Species Reactivity Database

Entrez Gene: 3684 Human

SwissProt: P11215 Human

Immunogen

A synthesized peptide derived from human CD11b aa1101-1152.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676578

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD11b Antibody (YA3423)
    Western blot analysis of extracts from TF-1(lane 2(20ug) , THP-1(lane 3(20ug) and U937(lane 4(20ug) using CD11b Antibody (HY-P83690) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD11b Antibody (YA3423)
    Flow cytometric analysis of 1X10^6 TF-1 cells labeling CD11b Antibody (YA3423) (HY-P83690, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD11b: Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog (s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M) .

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is mainly expressed in monocytes and granulocytes (PubMed: 1346576) . It is also expressed in neutrophils (protein level) (PubMed: 21193407) .

  • Isoforms & Post-Translational Modification

    CD11b has 2 isomers: P11215-1: 127179 Da (predicted) ; P11215-2: 127307 Da (predicted) .

  • Subunit

    Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD. Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance. Interacts with TMEM268; this interaction inhibits ITGAM degradation via the endosome-lysosome pathway.

  • SwissProt ID

    P11215

  • Gene ID
    3684 [NCBI]
  • Synonyms

    CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; CD11b; Leukocyte adhesion receptor MO1

  • Research Field

    Neuroscience

CD11b Antibody (YA3423) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from TF-1(lane 2(20ug) , THP-1(lane 3(20ug) and U937(lane 4(20ug) using CD11b Antibody (HY-P83690) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 TF-1 cells labeling CD11b Antibody (YA3423) (HY-P83690, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD11b Antibody (YA3423) Product Image
    1/16
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • CD11b Antibody (YA3423) Product Image
  • Experimental Validation Results for CD11b Antibody (YA3423)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from TF-1(lane 2(20ug) , THP-1(lane 3(20ug) and U937(lane 4(20ug) using CD11b Antibody (HY-P83690) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD11b Antibody (YA3423)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 TF-1 cells labeling CD11b Antibody (YA3423) (HY-P83690, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • Experimental Validation Results for CD11b Antibody (YA3423)
  • CD11b Antibody (YA3423) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from TF-1(lane 2(20ug) , THP-1(lane 3(20ug) and U937(lane 4(20ug) using CD11b Antibody (HY-P83690) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD11b Antibody (YA3423) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 TF-1 cells labeling CD11b Antibody (YA3423) (HY-P83690, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD11b Antibody (YA3423) Product Image WB
  • CD11b Antibody (YA3423) Product Image FC