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Cat. No.: HY-P81098

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CD34 Antibody

(Synonyms: CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.)

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Based on 1 Customer Validation

CD34 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD34.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, IHC-P, IHC-F, ICC/IF
Reactivity :
Human, Mouse

Formulation:
Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Conjugation:
Non-conjugated

ALL WB IHC-P

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Applications

Application	WB WB: Western Blot	IHC-P IHC-P: Immunohistochemistry-Paraffin	IHC-F IHC-F: Immunohistochemistry-Frozen	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence
Dilution Ratio	1:500-2000	1:100-500	1:100-500	1:100-500

Product Details

Description

CD34 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD34.

Host Rabbit
Clonality Polyclonal
Species Reactivity
Human, Mouse Predicted Reactivity: Rat,Dog,Pig,Cow,Rabbit

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
Observed Molecular Weight

Observed band size: 80-120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 39 kDa

Species Reactivity Database

Entrez Gene: 947 Human ; 12490 Mouse ; 305081 Rat

SwissProt: P28906 Human ; Q64314 Mouse ;

OMIM: 142230 Human

Immunogen

KLH conjugated synthetic peptide derived from human CD34: 301-385/385

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103058

Product Properties

Appearance

Solution
Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Verification Images

Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Mouse lung (lane 3(20μg)) 、Rat lung (lane 4(20μg)) and Rat heart (lane 5(20μg)) using CD34 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P81098, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Mouse Lung using CD34 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Mouse lung (lane 3(20μg)) 、Rat lung (lane 4(20μg)) and Rat heart (lane 5(20μg)) using CD34 Antibody (HY-P81098) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CD34 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81098, 1/250) , competitor's antibody (1/2500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CD34 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81098, 1/250) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD34 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81098, 1/250) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using CD34 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81098, 1/250) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using CD34 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81098, 1/250) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins
Subcellular Localization

Membrane; Single-pass type I membrane protein
Expression

Tissue_specificity:It is selectively expressed on hematopoietic progenitor cells and small vascular endothelial cells in various tissues.
Isoforms & Post-Translational Modification

P28906 has 2 isomers: P28906-1: 40716 Da (predicted); P28906-2: 35011 Da (predicted).
Highly glycosylated;Phosphorylated on serine residues by PKC
SwissProt ID

P28906
Gene ID

947 [NCBI]
Synonyms

CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.