CD34 Antibody

Cat. No.: HY-P81098: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CD34 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD34.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD34 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

CD34 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD34.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 80-120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse Predicted Reactivity: Rat,Dog,Pig,Cow,Rabbit

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P28906

Gene ID

947 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human CD34: 301-385/385

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Mouse Lung using CD34 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse heart (lane 2(20μg)) 、Mouse lung (lane 3(20μg)) 、Rat lung (lane 4(20μg)) and Rat heart (lane 5(20μg)) using CD34 Antibody (HY-P81098) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is selectively expressed on hematopoietic progenitor cells and small vascular endothelial cells in various tissues.

Isoforms & Post-Translational Modification：P28906 has 2 isomers: P28906-1: 40716 Da (predicted); P28906-2: 35011 Da (predicted).
Highly glycosylated;Phosphorylated on serine residues by PKC

RRID

AB_3103058

Database

Entrez Gene: 947 Human ; 12490 Mouse ; 305081 Rat

SwissProt: P28906 Human ; Q64314 Mouse ;

OMIM: 142230 Human

Synonyms

CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CD34 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD34 Antibody

CD34 Antibody Featured Recommendations:

Product Detail

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Background

Documentation

CD34 Antibody Related Classifications

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