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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Integrin alpha 6 Antibody (YA3020)

Integrin alpha 6 Antibody (YA3020)

Cat. No.: HY-P83275
COA
SDS
User Guide for Antibodies Technical Support

Integrin alpha 6 Antibody (YA3020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 6.

For research use only. We do not sell to patients.

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50 μL In-stock
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Documentation

Description

Integrin alpha 6 Antibody (YA3020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 6.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 127 kDa;
Observed band size: 127 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Integrin alpha 6 aa25-53.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using Integrin alpha 6 Antibody (HY-P83275) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464)
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor
Expression：
Tissue_specificity:Integrin α6/β4 is primarily expressed by epithelial cells. The isoform containing the X1 fragment is universally expressed. The isoform containing the X1X2 fragment is expressed in the heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and a few cell lines; they are always co-expressed with the universally present isoform containing the X1 fragment. In some tissues (e.g., salivary glands) , isoforms containing both cytoplasmic fragment A and fragment B can be detected, while in others, only an isoform containing one cytoplasmic fragment is found (fragment A in the epidermis, fragment B in the kidneys) . Processed integrin α6: expressed at low levels in normal prostate tissue, with elevated levels in prostate cancer tissue (protein level) (PubMed: 15023541) .
Isoforms & Post-Translational Modification：P23229 has 8 isomers: P23229-1: 126606 Da (predicted); P23229-2: 119476 Da (predicted); P23229-3: 122053 Da (predicted); P23229-4: 119088 Da (predicted); P23229-5: 121665 Da (predicted); P23229-6: 124029 Da (predicted); P23229-7: 106577 Da (predicted); P23229-9: 122358 Da (predicted).
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand;Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p) (PubMed:11359780, PubMed:15023541, PubMed:17303120). Production of alpha6p enhances prostate cancer cell invasion and migration (PubMed:17303120);Palmitoylation by DHHC3 enhances stability and cell surface expression
Subunit：Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond (By similarity). Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively (By similarity). ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with ADAM9 (By similarity). Interacts with RAB21 (PubMed:16754960). Interacts with MDK (PubMed:15466886). ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth (PubMed:15466886). Interacts with CD82; this interaction down-regulates ITGA6-mediated cell adhesion (PubMed:15557282)
RRID
Database

Entrez Gene: 3655 Human ; 16403 Mouse ; 81008 Rat

SwissProt: P23229 Human ; Q61739 Mouse ; Q63258 Rat

OMIM: 619817 Human

Research Field

Signal Transduction
Synonyms
ITGA6; CD49 antigen-like family member F; VLA-6; CD49f
Documentation
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Integrin alpha 6 Antibody (YA3020) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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