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Integrin alpha 6 Antibody (YA3020)

(Synonyms: ITGA6; CD49 antigen-like family member F; VLA-6; CD49f)
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Based on 1 publication(s) in Google Scholar

Integrin alpha 6 Antibody (YA3020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Integrin alpha 6 Antibody (YA3020)
  • Integrin alpha 6 Antibody (YA3020) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Integrin alpha 6 Antibody (YA3020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 6.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 127 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Integrin alpha 6 aa25-53.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105389

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin alpha 6 Antibody (YA3020)
    Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using Integrin alpha 6 Antibody (HY-P83275) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Integrin alpha 6 is an Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor

  • Expression


    Tissue_specificity:Integrin α6/β4 is primarily expressed by epithelial cells. The isoform containing the X1 fragment is universally expressed. The isoform containing the X1X2 fragment is expressed in the heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and a few cell lines; they are always co-expressed with the universally present isoform containing the X1 fragment. In some tissues (e.g., salivary glands) , isoforms containing both cytoplasmic fragment A and fragment B can be detected, while in others, only an isoform containing one cytoplasmic fragment is found (fragment A in the epidermis, fragment B in the kidneys) . Processed integrin α6: expressed at low levels in normal prostate tissue, with elevated levels in prostate cancer tissue (protein level) (PubMed: 15023541) .

  • Isoforms & Post-Translational Modification

    P23229 has 8 isomers: P23229-1: 126606 Da (predicted); P23229-2: 119476 Da (predicted); P23229-3: 122053 Da (predicted); P23229-4: 119088 Da (predicted); P23229-5: 121665 Da (predicted); P23229-6: 124029 Da (predicted); P23229-7: 106577 Da (predicted); P23229-9: 122358 Da (predicted).
    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand;Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p) (PubMed:11359780, PubMed:15023541, PubMed:17303120). Production of alpha6p enhances prostate cancer cell invasion and migration (PubMed:17303120);Palmitoylation by DHHC3 enhances stability and cell surface expression

  • Subunit

    Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond (By similarity). Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively (By similarity). ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3 (PubMed:20682778). ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (PubMed:22351760). ITGA6:ITGB4 interacts with IGF2 (PubMed:28873464). Interacts with ADAM9 (By similarity). Interacts with RAB21 (PubMed:16754960). Interacts with MDK (PubMed:15466886). ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth (PubMed:15466886). Interacts with CD82; this interaction down-regulates ITGA6-mediated cell adhesion (PubMed:15557282)

  • SwissProt ID

    P23229

  • Gene ID
    3655 [NCBI]
  • Synonyms

    ITGA6; CD49 antigen-like family member F; VLA-6; CD49f

  • Research Field

    Signal Transduction

Integrin alpha 6 Antibody (YA3020) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using Integrin alpha 6 Antibody (HY-P83275) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Integrin alpha 6 Antibody (YA3020) Product Image
    1/16
  • Experimental Validation Results for Integrin alpha 6 Antibody (YA3020)
  • Integrin alpha 6 Antibody (YA3020) Product Image
  • Experimental Validation Results for Integrin alpha 6 Antibody (YA3020)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using Integrin alpha 6 Antibody (HY-P83275) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin alpha 6 Antibody (YA3020)
  • Integrin alpha 6 Antibody (YA3020) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using Integrin alpha 6 Antibody (HY-P83275) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Integrin alpha 6 Antibody (YA3020) Product Image WB