Cytokeratin 10 Antibody (YA3784)(PBS only)

Cat. No.: HY-P84087A: Data Sheet User Guide for Antibodies
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Cytokeratin 10 Antibody (YA3784) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 10.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

Cytokeratin 10 Antibody (YA3784) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 10.

Host

Mouse

분자량

Predicted band size: 59 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey, Rat

SwissProt ID

P13645

Gene ID

3858 [NCBI]

Immunogen

Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity); (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization; (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur

Subcellular Localization：Secreted, extracellular space; Cell surface; Cytoplasm

Expression：
Tissue_specificity:It can be found in all cell layers above the basal layer, including the stratum corneum. It is expressed on the surface of lung cell lines (PubMed:19627498) . It is localized on the surface of exfoliated nasal epithelial cells (protein level) (PubMed:12427098) .

Induction:Repressed in keratinocytes by all-trans retinoic acid (ATRA) , via reduction of mRNA stability

Subunit：Heterotetramer of two type I and two type II keratins. Heterodimer with KRT1 (PubMed:24940650, PubMed:27595935). Two heterodimers of KRT1 and KRT10 form a heterotetramer (PubMed:27595935). The KRT10 subunit in the heterotetramer is probably disulfide-linked (PubMed:27595935). Interacts with PLEC isoform 1C, when in a heterodimer with KRT1 (PubMed:24940650)

Synonyms

BIE; EHK; K10; KPP; BCIE; CK10

각종 서류

User Guide for Antibodies (1077 KB)