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ATG10 Antibody (YA603)

(Synonyms: autophagy related 10; APG10; APG10L; pp12616)
Customer Review

Based on 1 Customer Validation

ATG10 Antibody (YA603) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG10.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATG10 Antibody (YA603)
  • ATG10 Antibody (YA603) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

ATG10 Antibody (YA603) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG10.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 83734 Human

SwissProt: Q9H0Y0 Human

Immunogen

Synthetic peptide corresponding to Human ATG10.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102608

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATG10 Antibody (YA603)
    Western blot analysis of extracts from K562 (lane 1) and 293T (lane 2) and Hela (lane 3) using ATG10 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    ATG10 is an E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q9H0Y0 has 2 isomers: Q9H0Y0-1: 25279 Da (predicted); Q9H0Y0-2: 14217 Da (predicted).

  • Subunit

    Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 (By similarity). Interacts with IRGM

  • SwissProt ID

    Q9H0Y0

  • Gene ID
    83734 [NCBI]
  • Synonyms

    autophagy related 10; APG10; APG10L; pp12616

  • Research Field

    Cell Biology

ATG10 Antibody (YA603) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and 293T (lane 2) and Hela (lane 3) using ATG10 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • ATG10 Antibody (YA603) Product Image
    1/16
  • Experimental Validation Results for ATG10 Antibody (YA603)
  • ATG10 Antibody (YA603) Product Image
  • Experimental Validation Results for ATG10 Antibody (YA603)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and 293T (lane 2) and Hela (lane 3) using ATG10 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for ATG10 Antibody (YA603)
  • ATG10 Antibody (YA603) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 1) and 293T (lane 2) and Hela (lane 3) using ATG10 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • ATG10 Antibody (YA603) Product Image WB