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FGF19 Antibody (YA2672)

(Synonyms: FGF19; Fibroblast growth factor 19)
Customer Review

Based on 1 Customer Validation

FGF19 Antibody (YA2672) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF19.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FGF19 Antibody (YA2672)
  • FGF19 Antibody (YA2672) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

FGF19 Antibody (YA2672) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF19.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 24/36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 24 kDa
Species Reactivity Database

Entrez Gene: 9965 Human

SwissProt: O95750 Human

Immunogen

A synthesized peptide derived from human FGF19 aa101-151/216.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105019

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FGF19 Antibody (YA2672)
    Western blot analysis of extracts from Hela(lane 2(20ug) ,HepG2(lane 3(20ug) and A549(lane 4(20ug) using FGF19 Antibody (HY-P82927) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    FGF19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed in fetal brain, cartilage, retina, and human gall bladder

    Induction:Induced by the bile acids receptor NR1H4 that binds and activates a NR1H4-responsive element within intron 2

  • Subunit

    Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL, KLB and heparan sulfate glycosaminoglycans that function as coreceptors.

  • SwissProt ID

    O95750

  • Gene ID
    9965 [NCBI]
  • Synonyms

    FGF19; Fibroblast growth factor 19

  • Research Field

    Signal Transduction

FGF19 Antibody (YA2672) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ,HepG2(lane 3(20ug) and A549(lane 4(20ug) using FGF19 Antibody (HY-P82927) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FGF19 Antibody (YA2672) Product Image
    1/16
  • Experimental Validation Results for FGF19 Antibody (YA2672)
  • FGF19 Antibody (YA2672) Product Image
  • Experimental Validation Results for FGF19 Antibody (YA2672)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) ,HepG2(lane 3(20ug) and A549(lane 4(20ug) using FGF19 Antibody (HY-P82927) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGF19 Antibody (YA2672)
  • FGF19 Antibody (YA2672) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) ,HepG2(lane 3(20ug) and A549(lane 4(20ug) using FGF19 Antibody (HY-P82927) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FGF19 Antibody (YA2672) Product Image WB