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Wnt2 Antibody (YA2973)

(Synonyms: Int 1 related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT2)
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Based on 1 Customer Validation

Wnt2 Antibody (YA2973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Wnt2 Antibody (YA2973)
  • Wnt2 Antibody (YA2973) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Wnt2 Antibody (YA2973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human WNT2 aa250-300/360.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wnt2 Antibody (YA2973)
    Western blot analysis of extracts from Jurkat (lane2, 20μg) using Wnt2 Antibody (HY-P83228). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Wnt2 functions within the Wnt/β-catenin signaling system, a conserved pathway that regulates development, adult tissue stem cells, and growth-related disease processes[1]. Mechanistically, Wnt2/2b signaling through canonical β-catenin specifies lung endoderm progenitors in the anterior foregut, and pathway activation can reprogram esophagus and stomach endoderm toward lung progenitor fate[2]. In lung mesenchyme, Wnt2 activates the airway smooth muscle program by regulating myocardin/Mrtf-B and Fgf10 expression[3]. In colorectal cancer cells, Wnt2 is highly expressed, co-expressed with Wnt/β-catenin target genes, and required for target-gene expression and cell proliferation[4]. Compared with related isoforms, WNT2B is a WNT2 paralogue that encodes WNT2B1 and WNT2B2 through alternative splicing and alternative promoter usage[5]. For experimental applications, Wnt2 loss-of-function, Wnt2 neutralization, β-catenin deletion, Dkk1 expression, and multi-Frizzled antibody blockade provide practical approaches to test ligand-dependent Wnt signaling in developmental and cancer models[2][4][6].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix; Secreted

  • Expression


    Tissue_specificity:Expressed in brain in the thalamus, in human lung and in placenta

  • SwissProt ID

    P09544

  • Gene ID
    11197 [NCBI]
  • Synonyms

    Int 1 related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT2

  • Research Field

    Stem Cells

[1]. Nusse R, et al. Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities. Cell. 2017 Jun 1;169(6):985-999. [Content Brief]

[2]. Goss AM, et al. Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. Dev Cell. 2009 Aug;17(2):290-8. [Content Brief]

[3]. Paix A, et al. Bi-polarized translation of ascidian maternal mRNA determinant pem-1 associated with regulators of the translation machinery on cortical Endoplasmic Reticulum (cER). Dev Biol. 2011 Sep 1;357(1):211-26. [Content Brief]

[4]. Jung YS, et al. Wnt2 complements Wnt/β-catenin signaling in colorectal cancer. Oncotarget. 2015 Nov 10;6(35):37257-68. [Content Brief]

[5]. Katoh M. Differential regulation of WNT2 and WNT2B expression in human cancer. Int J Mol Med. 2001 Dec;8(6):657-60. doi: 10.3892/ijmm.8.6.657. PMID: 11712082. et al. Differential regulation of WNT2 and WNT2B expression in human cancer. Int J Mol Med. 2001 Dec;8(6):657-60. [Content Brief]

[6]. Zhang Y, et al. Canonical wnt signaling is required for pancreatic carcinogenesis. Cancer Res. 2013 Aug 1;73(15):4909-22. [Content Brief]

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Wnt2 Antibody (YA2973) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg) using Wnt2 Antibody (HY-P83228). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Wnt2 Antibody (YA2973) Product Image
    1/16
  • Experimental Validation Results for Wnt2 Antibody (YA2973)
  • Wnt2 Antibody (YA2973) Product Image
  • Experimental Validation Results for Wnt2 Antibody (YA2973)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg) using Wnt2 Antibody (HY-P83228). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wnt2 Antibody (YA2973)
  • Wnt2 Antibody (YA2973) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane2, 20μg) using Wnt2 Antibody (HY-P83228). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Wnt2 Antibody (YA2973) Product Image WB