MARCH7 Antibody
(Synonyms: AXOT, MARCH7, RNF177, MARCH-VII, Axotrophin, Membrane-associated RING finger protein 7, RING finger protein 177, RING-type E3 ubiquitin transferase MARCHF7)
Based on 1 Customer Validation
MARCH7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MARCH7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF
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Reactivity :
Human, Rat
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Formulation:
Supplied in 0.01M TBS (PH7.4), with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
MARCH7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MARCH7.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Rat Predicted Reactivity: Mouse,Rabbit,Pig,Cow,Sheep,Dog,Horse
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 74 kDa & 78 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 78 kDa
KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin: 31-130/704.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (PH7.4), with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
2026-03-07 00:00:00 is an E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in T-cell proliferation by regulating LIF secretion (By similarity). May play a role in lysosome homeostasis. Promotes 'Lys-6', 'Lys-11' and 'Lys-63'-linked mixed polyubiquitination on ATG14 leading to the inhibition of autophagy by impairing the interaction between ATG14 and STX7. Participates in the dopamine-mediated negative regulation of the NLRP3 inflammasome by promoting its ubiquitination and subsequent degradation[1][2][3][4].
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Subcellular Localization
Cytoplasm
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Isoforms & Post-Translational Modification
2026-03-07 00:00:00 has 2 isoforms, Q9H992-1: amino acid length is 704, molecular weight is 78051 Da (predicted); Q9H992-2: amino acid length is 666, molecular weight is 73778 Da (predicted).2026-03-07 00:00:00 存在 2 个异构体，Q9H992-1：氨基酸个数为 704 个，分子量为 78051 Da (预测)；Q9H992-2：氨基酸个数为 666 个，分子量为 73778 Da (预测)。
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SwissProt ID
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Synonyms
AXOT, MARCH7, RNF177, MARCH-VII, Axotrophin, Membrane-associated RING finger protein 7, RING finger protein 177, RING-type E3 ubiquitin transferase MARCHF7
Documentation
[1]. Flierman D, et al. E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11589-94. [Content Brief]
[2]. Lenk GM, et al. CRISPR knockout screen implicates three genes in lysosome function. Sci Rep. 2019 Jul 3;9(1):9609. [Content Brief]
[3]. Shi X, et al. MARCH7-mediated ubiquitination decreases the solubility of ATG14 to inhibit autophagy. Cell Rep. 2023 Sep 26;42(9):113045. [Content Brief]
[4]. Yan Y, et al. Dopamine controls systemic inflammation through inhibition of NLRP3 inflammasome. Cell. 2015 Jan 15;160(1-2):62-73. [Content Brief]
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