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Thrombospondin 1 Antibody (YA3553)

(Synonyms: TSP; THBS; TSP1; TSP-1; THBS-1)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Thrombospondin 1 Antibody (YA3553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombospondin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Thrombospondin 1 Antibody (YA3553) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:2000-1:8000 1:100 1:500 1:1000

Product Details

Description

Thrombospondin 1 Antibody (YA3553) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombospondin 1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 170 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 129 kDa
Immunogen

Synthetic peptide within human Thrombospondin 1 aa 500-1,170 / 1,170.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718744

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Western blot analysis of extracts from HUVEC (lane 2(40μg) and HUVEC (lane 3(60μg) using Thrombospondin 1(HY-P83750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Flow cytometric analysis of 1X10^6 NIH-3T3 cells labeling Thrombospondin 1 Antibody (YA3553) (HY-P83750, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Immunocytochemistry analysis of NIH3T3 a cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    Immunocytochemistry analysis of HUVEC cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Thrombospondin 1 is an Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis. Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis. Ligand for receptor CD47. Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure. Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS. Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4. Negatively modulates wound healing, acting via CD47. Ligand for receptor CD36. Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36. Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47. Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner. Ligand for immunoglobulin-like cell surface receptor SIRPA. Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA. Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. May be involved in age-related conditions, including metabolic dysregulation, during normal aging[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Secreted; Cell surface; Secreted, extracellular space, extracellular matrix; Endoplasmic reticulum; Sarcoplasmic reticulum

  • Expression


    Tissue_specificity:Platelet expression (protein level) (PubMed: 101549) . Expression (protein level) in immature and mature dendritic cells derived from monocytes (PubMed: 14568985) .

    Induction:Expression is induced by PGE2, S.aureus and lipopolysaccharide (PubMed:14568985) . Induced in arteries and lung parenchyma following injury or stress (PubMed:27742621) . Expression in vasculature, including arteries, increases in normal aging (PubMed:29042481, PubMed:32679764)

    Positive sample: HUVEC cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, NIH/3T3, HUVEC, human bone marrow tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.

  • Isoforms & Post-Translational Modification

    P07996 has 2 isomers: P07996-1: 129383 Da (predicted); P07996-2: 120148 Da (predicted).

  • Subunit

    Homotrimer; disulfide-linked (PubMed:101549, PubMed:18285447). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3 (PubMed:2478219, PubMed:6489349, PubMed:6693501). Binds heparin (PubMed:101549, PubMed:16407063, PubMed:18065761). Interacts (via the C-terminal domain) with CD47 (PubMed:19004835, PubMed:8550562). Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect (PubMed:10613822, PubMed:1371676). Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (PubMed:11134179). Interacts with ATF6 (via lumenal domain) (By similarity). Interacts with FN1; this interaction is enhanced by TNFAIP6, which may act as a bridging molecule between FN1 and THBS1 (PubMed:18042364). Interacts with SIRPA; the interaction stimulates phosphorylation of SIRPA (PubMed:24511121)

  • SwissProt ID

    P07996

  • Gene ID
    7057 [NCBI]
  • Synonyms

    TSP; THBS; TSP1; TSP-1; THBS-1

Thrombospondin 1 Antibody (YA3553) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(40μg) and HUVEC (lane 3(60μg) using Thrombospondin 1(HY-P83750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 NIH-3T3 cells labeling Thrombospondin 1 Antibody (YA3553) (HY-P83750, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH3T3 a cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thrombospondin 1 Antibody (YA3553) Product Image
    1/16
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Thrombospondin 1 Antibody (YA3553) Product Image
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(40μg) and HUVEC (lane 3(60μg) using Thrombospondin 1(HY-P83750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 NIH-3T3 cells labeling Thrombospondin 1 Antibody (YA3553) (HY-P83750, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH3T3 a cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Experimental Validation Results for Thrombospondin 1 Antibody (YA3553)
  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HUVEC (lane 2(40μg) and HUVEC (lane 3(60μg) using Thrombospondin 1(HY-P83750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Thrombospondin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 NIH-3T3 cells labeling Thrombospondin 1 Antibody (YA3553) (HY-P83750, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH3T3 a cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thrombospondin 1 Antibody (YA3553) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HUVEC cells labeling Thrombospondin 1 with Thrombospondin 1 Antibody (YA3553) (HY-P83750) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Thrombospondin 1 Antibody (YA3553) ((HY-P83750) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thrombospondin 1 Antibody (YA3553) Product Image WB
  • Thrombospondin 1 Antibody (YA3553) Product Image IHC-P
  • Thrombospondin 1 Antibody (YA3553) Product Image IHC-P
  • Thrombospondin 1 Antibody (YA3553) Product Image FC
  • Thrombospondin 1 Antibody (YA3553) Product Image ICC
  • Thrombospondin 1 Antibody (YA3553) Product Image ICC