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PGP9.5 Antibody (YA231)

(Synonyms: UCHL1 / PGP9.5; UCHL1; B220; CD 45; CD45; cd45 antigen; ec3.1.3.48; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; L-CA; lca; Leukocyte common antigen; LY 5; LY5; Protein tyrosine phosphatase receptor type )
Customer Review

Based on 1 Customer Validation

PGP9.5 Antibody (YA231) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGP9.5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • PGP9.5 Antibody (YA231) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:200-1:1000 1:200-1:500 1:50-1:100 1:10-1:100

Product Details

Description

PGP9.5 Antibody (YA231) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGP9.5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human PGP9.5.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102974

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    Western blot analysis of extracts from U-87 MG (lane2(20μg), SH-SY5Y (lane3(20μg), A549 (lane4(20μg) and C6 (lane5(20μg) using PGP9.5 Antibody (HY-P80785). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526)

  • Subcellular Localization

    Cytoplasm; Endoplasmic reticulum membrane; Lipid-anchor

  • Expression


    Tissue_specificity:It has been found in both the cell bodies and processes of neocortical neurons (protein level) . It is expressed in neurons and cells of the diffuse neuroendocrine system and in tumors therein. Expression is weak in the ovary. It is downregulated in the brain tissue of Parkinson's disease and Alzheimer's disease.

  • Isoforms & Post-Translational Modification

    P09936 has 3 isomers: P09936-1: 24824 Da (predicted); P09936-2: 24227 Da (predicted); P09936-3: 23513 Da (predicted).
    O-glycosylated

  • Subunit

    Monomer. Homodimer. Interacts with COPS5

  • SwissProt ID

    P09936

  • Gene ID
    7345 [NCBI]
  • Synonyms

    UCHL1 / PGP9.5; UCHL1; B220; CD 45; CD45; cd45 antigen; ec3.1.3.48; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; L-CA; lca; Leukocyte common antigen; LY 5; LY5; Protein tyrosine phosphatase receptor type

  • Research Field

    Neuroscience

PGP9.5 Antibody (YA231) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U-87 MG (lane2(20μg), SH-SY5Y (lane3(20μg), A549 (lane4(20μg) and C6 (lane5(20μg) using PGP9.5 Antibody (HY-P80785). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGP9.5 Antibody (YA231) Product Image
    1/16
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • PGP9.5 Antibody (YA231) Product Image
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U-87 MG (lane2(20μg), SH-SY5Y (lane3(20μg), A549 (lane4(20μg) and C6 (lane5(20μg) using PGP9.5 Antibody (HY-P80785). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • Experimental Validation Results for PGP9.5 Antibody (YA231)
  • PGP9.5 Antibody (YA231) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U-87 MG (lane2(20μg), SH-SY5Y (lane3(20μg), A549 (lane4(20μg) and C6 (lane5(20μg) using PGP9.5 Antibody (HY-P80785). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • PGP9.5 Antibody (YA231) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGP9.5 Antibody (YA231) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PGP9.5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGP9.5 Antibody (YA231) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGP9.5 Antibody (YA231) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 Antibody (HY-P80785) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PGP9.5 Antibody (HY-P80785) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGP9.5 Antibody (YA231) Product Image WB
  • PGP9.5 Antibody (YA231) Product Image IHC-P
  • PGP9.5 Antibody (YA231) Product Image IHC-P
  • PGP9.5 Antibody (YA231) Product Image ICC
  • PGP9.5 Antibody (YA231) Product Image ICC