Phospho-Hormone sensitive lipase (Ser660) Antibody (YA9820)
(Synonyms: LIPE; Hormone-sensitive lipase; HSL)
Based on 1 Customer Validation
Phospho-Hormone sensitive lipase (Ser660) Antibody (YA9820) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser660).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:2000 |
Product Details
Phospho-Hormone sensitive lipase (Ser660) Antibody (YA9820) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser660).
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 84 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 117 kDa
Synthetic phospho-peptide corresponding to residues surrounding Ser660 of Human HSL.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Hormone sensitive lipase is a Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity)[1][2][3][4].
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Subcellular Localization
Cell membrane; Membrane, caveola; Cytoplasm, cytosol; Lipid droplet
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Expression
Tissue_Specificity: Testis. -
Isoforms & Post-Translational Modification
Phospho-Hormone sensitive lipase has 2 isoforms, Q05469-1: amino acid length is 1076, molecular weight is 116598 Da (predicted); Q05469-2: amino acid length is 775, molecular weight is 84128 Da (predicted).
Phosphorylation by AMPK reduces its translocation towards the lipid droplets. -
Subunit
Monomer and homodimer (By similarity).
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SwissProt ID
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Synonyms
LIPE; Hormone-sensitive lipase; HSL
Documentation
References
[1]. Ali YB, et al. Continuous monitoring of cholesterol oleate hydrolysis by hormone-sensitive lipase and other cholesterol esterases. J Lipid Res. 2005 May;46(5):994-1000. [Content Brief]
[2]. Gao J, et al. Identification of a novel keratinocyte retinyl ester hydrolase as a transacylase and lipase. J Invest Dermatol. 2005 Jun;124(6):1259-66. [Content Brief]
[3]. Rodriguez JA, et al. In vitro stereoselective hydrolysis of diacylglycerols by hormone-sensitive lipase. Biochim Biophys Acta. 2010 Jan;1801(1):77-83. [Content Brief]
[4]. Holst LS, et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics. 1996 Aug 1;35(3):441-7. [Content Brief]
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