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TRAP Antibody (YA2321)

(Synonyms: TRAP; SPENCDI; TrATPase)
Customer Review

Based on 1 Customer Validation

TRAP Antibody (YA2321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRAP Antibody (YA2321)
  • TRAP Antibody (YA2321) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

TRAP Antibody (YA2321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAP.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35,42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase aa150-325.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRAP Antibody (YA2321)
    Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Jurkat (lane 2, 20 μg), C6 (lane 3, 20 μg), RAW264.7 (lane 4, 20 μg), PC-12 (lane 5, 20 μg), and 3T3 (lane 6, 20 μg) using TR AP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TRAP is involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias

  • Subcellular Localization

    Lysosome

  • Subunit

    Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s)

  • SwissProt ID

    P13686

  • Gene ID
    54 [NCBI]
  • Synonyms

    TRAP; SPENCDI; TrATPase

  • Research Field

    Immunology

TRAP Antibody (YA2321) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Jurkat (lane 2, 20 μg), C6 (lane 3, 20 μg), RAW264.7 (lane 4, 20 μg), PC-12 (lane 5, 20 μg), and 3T3 (lane 6, 20 μg) using TR AP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRAP Antibody (YA2321) Product Image
    1/16
  • Experimental Validation Results for TRAP Antibody (YA2321)
  • TRAP Antibody (YA2321) Product Image
  • Experimental Validation Results for TRAP Antibody (YA2321)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Jurkat (lane 2, 20 μg), C6 (lane 3, 20 μg), RAW264.7 (lane 4, 20 μg), PC-12 (lane 5, 20 μg), and 3T3 (lane 6, 20 μg) using TR AP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRAP Antibody (YA2321)
  • TRAP Antibody (YA2321) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Jurkat (lane 2, 20 μg), C6 (lane 3, 20 μg), RAW264.7 (lane 4, 20 μg), PC-12 (lane 5, 20 μg), and 3T3 (lane 6, 20 μg) using TR AP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRAP Antibody (YA2321) Product Image WB