TRAP Antibody (YA2321)

Art. -Nr.: HY-P82576: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TRAP Antibody (YA2321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAP.

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TRAP Antibody (YA2321) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

TRAP Antibody (YA2321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAP.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molekulargewicht

Predicted band size: 37 kDa;

Observed band size: 35,42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P13686

Gene ID

54 [NCBI]

Immunogen

A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase aa150-325.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Reinheit	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Jurkat (lane 2, 20 μg), C6 (lane 3, 20 μg), RAW264.7 (lane 4, 20 μg), PC-12 (lane 5, 20 μg), and 3T3 (lane 6, 20 μg) using TR AP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias

Subcellular Localization：Lysosome

Subunit：Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s)

Database

Entrez Gene: 54 Human ; 11433 Mouse ; 25732 Rat

SwissProt: P13686 Human ; Q05117 Mouse ; P29288 Rat

OMIM: 607944 Human

Research Field

Immunology

Synonyms

TRAP; SPENCDI; TrATPase

Dokumentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TRAP Antibody (YA2321) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TRAP Antibody (YA2321)

TRAP Antibody (YA2321) Spezialitätsempfehlungen:

Product Detail

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Background

Beschreibung

TRAP Antibody (YA2321) Related Classifications

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