alpha 1a Adrenergic Receptor Antibody (YA2059)(PBS only)

Cat. No.: HY-P82314A: Data Sheet User Guide for Antibodies
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alpha 1a Adrenergic Receptor Antibody (YA2059) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha 1a Adrenergic Receptor.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

alpha 1a Adrenergic Receptor Antibody (YA2059) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha 1a Adrenergic Receptor.

Host

Rabbit

Molecular Weight

Predicted band size: 51 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35348

Gene ID

148 [NCBI]

Immunogen

A synthetic peptide of human alpha 1a Adrenergic Receptor

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
FC FC: Flow Cytometry	1:100-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes

Subcellular Localization：Nucleus membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cytoplasm; Membrane, caveola

Expression：
Tissue_specificity:Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart

Isoforms & Post-Translational Modification：P35348 has 9 isomers: P35348-1: 51487 Da (predicted); P35348-2: 52205 Da (predicted); P35348-3: 47461 Da (predicted); P35348-4: 50777 Da (predicted); P35348-5: 32433 Da (predicted); P35348-6: 37468 Da (predicted); P35348-7: 35829 Da (predicted); P35348-8: 40725 Da (predicted); P35348-9: 40638 Da (predicted).
C-terminal Ser or Thr residues may be phosphorylated

Subunit：Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes (PubMed:22120526). Interacts with CAVIN4 (PubMed:24567387)

Research Field

Neuroscience

Synonyms

ADRA1A; ADRA1C; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1A adrenoceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c

Documentation

User Guide for Antibodies (1077 KB)