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IDH2 Antibody (YA3065)

(Synonyms: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH)
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Based on 1 Customer Validation

IDH2 Antibody (YA3065) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IDH2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IDH2 Antibody (YA3065)
  • IDH2 Antibody (YA3065) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

IDH2 Antibody (YA3065) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IDH2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human IDH2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IDH2 Antibody (YA3065)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), Rat lung (lane 4), and Rat liver (lane 5) using IDH2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    IDH2 (isocitrate dehydrogenase 2) encodes a mitochondrial NADP+-dependent isocitrate dehydrogenase that catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate while generating NADPH, thereby supporting mitochondrial metabolism and redox homeostasis[1][2]. Mechanistically, IDH2 participates in intermediary metabolism through the conversion of isocitrate to 2-oxoglutarate, linking cellular energy metabolism with NADPH production required for biosynthetic and antioxidant processes[2]. In disease settings, recurrent IDH2 mutations confer a neomorphic enzymatic activity that promotes the production of the oncometabolite 2-hydroxyglutarate (2-HG), which is associated with epigenetic dysregulation and impaired cellular differentiation in malignancies, particularly acute myeloid leukemia (AML) [3][4][5]. Therefore, mutant IDH2 has become an important molecular target in cancer research and precision oncology[3][5]. Compared with related isoforms, IDH1 and IDH2 both utilize NADP+ and catalyze the same biochemical reaction, whereas IDH2 is localized to mitochondria, distinguishing its metabolic context from the cytosolic IDH1 isoform[2][5]. For experimental and translational applications, enasidenib is a selective small-molecule inhibitor of mutant IDH2 that reduces 2-HG levels and promotes myeloid differentiation, providing a widely used pharmacological tool for investigating mutant IDH2 biology and therapeutic response mechanisms in AML models[3][5][6].

  • Subcellular Localization

    Mitochondrion

  • Isoforms & Post-Translational Modification

    P48735 has 2 isomers: P48735-1: 50909 Da (predicted); P48735-2: 45180 Da (predicted).
    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3

  • Subunit

    Homodimer

  • SwissProt ID

    P48735

  • Gene ID
    3418 [NCBI]
  • Synonyms

    IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH

  • Research Field

    Signal Transduction

[1]. IDH2 gene information from NCBI.

[2]. Cadoux-Hudson T, et al. Isocitrate dehydrogenase gene variants in cancer and their clinical significance. Biochem Soc Trans. 2021;49(6):2561-2572.

[3]. Amaya ML, et al. Targeting the IDH2 Pathway in Acute Myeloid Leukemia. Clin Cancer Res. 2018 Oct 15;24(20):4931-4936. [Content Brief]

[4]. Guo J, et al. Biological Roles and Therapeutic Applications of IDH2 Mutations in Human Cancer. Front Oncol. 2021 Apr 26;11:644857. [Content Brief]

[5]. Wikipedia.

[6]. Wikipedia.

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IDH2 Antibody (YA3065) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), Rat lung (lane 4), and Rat liver (lane 5) using IDH2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IDH2 Antibody (YA3065) Product Image
    1/16
  • Experimental Validation Results for IDH2 Antibody (YA3065)
  • IDH2 Antibody (YA3065) Product Image
  • Experimental Validation Results for IDH2 Antibody (YA3065)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), Rat lung (lane 4), and Rat liver (lane 5) using IDH2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for IDH2 Antibody (YA3065)
  • IDH2 Antibody (YA3065) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), Rat lung (lane 4), and Rat liver (lane 5) using IDH2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IDH2 Antibody (YA3065) Product Image WB