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DARPP32 Antibody (YA5816)

(Synonyms: PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein)
Customer Review

Based on 1 Customer Validation

DARPP32 Antibody (YA5816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DARPP32.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DARPP32 Antibody (YA5816)
  • DARPP32 Antibody (YA5816) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

DARPP32 Antibody (YA5816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DARPP32.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DARPP32 Antibody (YA5816)
    Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using DARPP-32 Antibody (HY-P86124) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    DARPP32 is an Inhibitor of protein-phosphatase 1

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q9UD71 has 2 isomers: Q9UD71-1: 22963 Da (predicted); Q9UD71-2: 18738 Da (predicted).
    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein;Phosphorylation of Thr-34 is required for activity

  • SwissProt ID

    Q9UD71

  • Gene ID
    84152 [NCBI]
  • Synonyms

    PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

DARPP32 Antibody (YA5816) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using DARPP-32 Antibody (HY-P86124) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • DARPP32 Antibody (YA5816) Product Image
    1/16
  • Experimental Validation Results for DARPP32 Antibody (YA5816)
  • DARPP32 Antibody (YA5816) Product Image
  • Experimental Validation Results for DARPP32 Antibody (YA5816)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using DARPP-32 Antibody (HY-P86124) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DARPP32 Antibody (YA5816)
  • DARPP32 Antibody (YA5816) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse brain (lane 1(20μg)) and Rat brain (lane 2(20μg)) using DARPP-32 Antibody (HY-P86124) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • DARPP32 Antibody (YA5816) Product Image WB