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Napsin A Antibody (YA5541)

(Synonyms: Napsin-A; Aspartyl protease 4; ASP4; Asp 4; Napsin-1; TA01/TA02; )
Customer Review

Based on 1 Customer Validation

Napsin A Antibody (YA5541) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Napsin A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Napsin A Antibody (YA5541) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

Napsin A Antibody (YA5541) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Napsin A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

Synthesized peptide derived from human Napsin A. AA range: 100-200

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719053

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Napsin A Antibody (YA5541)
    Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Napsin A Antibody (YA5541)
    Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Napsin A may be involved in processing of pneumocyte surfactant precursors

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is mainly expressed in human lungs (type II alveolar cells) and kidneys, as well as fetal lungs. Its content is low in human spleen and extremely low in peripheral blood leukocytes.

  • SwissProt ID

    O96009

  • Gene ID
    9476 [NCBI]
  • Synonyms

    Napsin-A; Aspartyl protease 4; ASP4; Asp 4; Napsin-1; TA01/TA02;

Napsin A Antibody (YA5541) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Napsin A Antibody (YA5541) Product Image
    1/16
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Napsin A Antibody (YA5541) Product Image
  • Experimental Validation Results for Napsin A Antibody (YA5541)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Napsin A Antibody (YA5541)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Experimental Validation Results for Napsin A Antibody (YA5541)
  • Napsin A Antibody (YA5541) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Napsin A Antibody (YA5541) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Napsin A with Napsin A Antibody (HY-P85849) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Napsin A Antibody (HY-P85849) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Napsin A Antibody (YA5541) Product Image ICC
  • Napsin A Antibody (YA5541) Product Image ICC