IL1F6 (IL36 alpha) Antibody (YA7837)
(Synonyms: FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1(EPSILON); IL1F6)
Based on 1 Customer Validation
IL1F6 (IL36 alpha) Antibody (YA7837) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL1F6 (IL36 alpha).
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|
| Dilution Ratio | 1:2000 | 1:100 | 1:100 |
Product Details
IL1F6 (IL36 alpha) Antibody (YA7837) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL1F6 (IL36 alpha).
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 17.5 kDa
Full length human recombinant protein of human IL1F6 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IL-36 alpha, a subform of IL-36 family, belongs to IL-1 superfamily. Human IL-36 alpha shares <55% aa sequence identity with mouse. IL-36 alpha binds to IL-36R and activates NF-κB and MAPK signaling pathways, thereby mediating inflammatory response. But the activation requires N-terminal cleavage by neutrophil granule-derived proteases, such as cathepsin G, elastase and proteinase-3[1]. IL-36 alpha can also bind IL-1Rrp2 and recruit IL-1RAcP. IL-36 alpha activats the MAPK, Erk1/2 and JNK through IL-36R/IL-1RAcP[2]. IL-36 alpha is expressed in monocytes, T/B-lymphocytes, spleen, bone-marrow tonsils, lymph nodes and skin[2]. IL-36 alpha is up-regulated in injured kidney. IL-36 alpha is associated with the development of renal pathologies, as well as hepatocellular carcinoma, and some inflammatory/immune diseases[3].
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Subcellular Localization
Cytoplasm,Secreted
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Expression
Tissue_Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin
Induction: By Il-1 and TNF-alpha -
Isoforms & Post-Translational Modification
IL-36 alpha: 158 amino acids, molecular weight 17684 Da.
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Subunit
Interacts with TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion
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SwissProt ID
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Synonyms
FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1(EPSILON); IL1F6
Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210. [Content Brief]
[2]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65. [Content Brief]
[3]. Murrieta-Coxca JM, et al. IL-36 Cytokines: Regulators of Inflammatory Responses and Their Emerging Role in Immunology of Reproduction. Int J Mol Sci. 2019 Apr 3;20(7):1649. [Content Brief]
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