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Gelsolin Antibody (YA1570)

(Synonyms: ADF; AGEL; Brevin; Gelsolin; Gsn)
Customer Review

Based on 1 Customer Validation

Gelsolin Antibody (YA1570) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gelsolin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Gelsolin Antibody (YA1570) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50-1:100

Product Details

Description

Gelsolin Antibody (YA1570) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gelsolin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 86 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Gelsolin aa450-485.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human pancreas using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded human soft tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Gelsolin is a Ca2+-dependent actin-binding protein that plays a crucial role in processes such as cytoskeleton remodeling and cell migration. Under the regulation of calcium ions, Gelsolin can sever and cap actin filaments, regulate the assembly and disassembly of actin filaments, and remodel the cytoskeleton. Gelsolin is also involved in cell movement, affecting the migration of fibroblasts and the phagocytosis of immune cells, and can regulate the growth cones and filopodia of neurons. In addition, as a downstream effector molecule of Rac, Gelsolin can be dissociated upon activation by Rac, promoting actin remodeling and influencing cellular physiological activities. Mutant mice lacking Gelsolin and clinical studies have shown that Gelsolin is associated with a number of conditions such as inflammation, cancer, and amyloidosis[1].

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Secreted

  • Expression


    Tissue_specificity:Phagocytes, platelets, fibroblasts, non-muscle cells, smooth muscle cells, and skeletal muscle cells

  • Isoforms & Post-Translational Modification

    P06396 has 4 isomers: P06396-1: 85698 Da (predicted); P06396-2: 80641 Da (predicted); P06396-3: 81941 Da (predicted); P06396-4: 81485 Da (predicted).
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids

  • Subunit

    Binds to actin and to fibronectin. Identified in a complex composed of ACTA1, COBL, GSN and TMSB4X.

  • SwissProt ID

    P06396

  • Gene ID
    2934 [NCBI]
  • Synonyms

    ADF; AGEL; Brevin; Gelsolin; Gsn

  • Research Field

    Signal Transduction

References

[1]. Spinardi L, et al. Gelsolin and diseases. Subcell Biochem. 2007;45:55-69. [Content Brief]

Gelsolin Antibody (YA1570) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Gelsolin Antibody (YA1570) Product Image
    1/16
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Gelsolin Antibody (YA1570) Product Image
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Experimental Validation Results for Gelsolin Antibody (YA1570)
  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human soft tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using Gelsolin Antibody (HY-P81825, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Gelsolin Antibody (YA1570) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling Gelsolin Antibody (HY-P81825, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image IHC-P
  • Gelsolin Antibody (YA1570) Product Image FC
  • Gelsolin Antibody (YA1570) Product Image FC