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GPD2 Antibody (YA2958)

(Synonyms: GDH2; GPDM; mGPDH)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

GPD2 Antibody (YA2958) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPD2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GPD2 Antibody (YA2958)
  • GPD2 Antibody (YA2958) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) GPD2 Antibody (YA2958)

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

GPD2 Antibody (YA2958) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPD2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human GPD2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105027

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GPD2 Antibody (YA2958)
    Western blot analysis of extracts from Hela (lane2, 20μg), MCF-7 (lane3, 20μg) and THP-1 (lane4, 20μg) using GPD2 Antibody (HY-P83213). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GPD2 is a Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device

  • Subcellular Localization

    Mitochondrion

  • Isoforms & Post-Translational Modification

    P43304 has 2 isomers: P43304-1: 80853 Da (predicted); P43304-2: 67531 Da (predicted).

  • SwissProt ID

    P43304

  • Gene ID
    2820 [NCBI]
  • Synonyms

    GDH2; GPDM; mGPDH

  • Research Field

    Tags & Cell Markers

GPD2 Antibody (YA2958) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), MCF-7 (lane3, 20μg) and THP-1 (lane4, 20μg) using GPD2 Antibody (HY-P83213). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GPD2 Antibody (YA2958) Product Image
    1/16
  • Experimental Validation Results for GPD2 Antibody (YA2958)
  • GPD2 Antibody (YA2958) Product Image
  • Experimental Validation Results for GPD2 Antibody (YA2958)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), MCF-7 (lane3, 20μg) and THP-1 (lane4, 20μg) using GPD2 Antibody (HY-P83213). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GPD2 Antibody (YA2958)
  • GPD2 Antibody (YA2958) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), MCF-7 (lane3, 20μg) and THP-1 (lane4, 20μg) using GPD2 Antibody (HY-P83213). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GPD2 Antibody (YA2958) Product Image WB