ARFGEF2 Antibody (YA2146)(PBS only)

Cat. No.: HY-P82401A: Data Sheet User Guide for Antibodies
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ARFGEF2 Antibody (YA2146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ARFGEF2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ARFGEF2 Antibody (YA2146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ARFGEF2.

Host

Rabbit

Molecular Weight

Predicted band size: 202 kDa;

Observed band size: 202 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y6D5

Gene ID

10564 [NCBI]

Immunogen

A synthetic peptide of human ARFGEF2 aa1501-1514.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways

Subcellular Localization：Cytoplasm; Membrane; Golgi apparatus; Cytoplasm, perinuclear region; Golgi apparatus, trans-Golgi network; Endosome; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cell projection, dendrite; Cytoplasmic vesicle; Synapse; Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:Expressed in placenta, lung, heart, brain, kidney and pancreas

Subunit：Homodimer (Probable). Interacts with ARFGEF1/BIG1; both proteins are probably part of the same or very similar macromolecular complexes.

Research Field

Signal Transduction

Synonyms

BIG2; PVNH2; dJ1164I10.1

Documentation

User Guide for Antibodies (1077 KB)