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MAP4K1 Antibody (YA4410)

(Synonyms: HPK1)

MAP4K1 Antibody (YA4410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP4K1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Monkey, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MAP4K1 Antibody (YA4410)
  • MAP4K1 Antibody (YA4410) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MAP4K1 Antibody (YA4410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP4K1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey, Rat
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 92 kDa
Immunogen

Purified recombinant fragment of human MAP4K1 (AA:278-477) expressed in mammalian.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAP4K1 Antibody (YA4410)
    Flow cytometric analysis of 1X10^6 Ramos cells labeling MAP4K1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MAP4K1 is a Serine/threonine-protein kinase, which plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. May play a role in hematopoietic lineage decisions and growth regulation. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production[1][2][3].

  • Expression


    Tissue_specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine

  • Isoforms & Post-Translational Modification

    Q92918 has 2 isomers: Q92918-1: 91296 Da (predicted); Q92918-2: 90425 Da (predicted).
    Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome;Tyrosine-phosphorylated after activation of hemopoietic cells;Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome

  • Subunit

    Interacts with MAP3K1 (PubMed:8824585). Interacts with FBXW8 (PubMed:24362026). Interacts with CLNK (via its SH2 domain) (By similarity)

  • SwissProt ID

    Q92918

  • Gene ID
    11184 [NCBI]
  • Synonyms

    HPK1

MAP4K1 Antibody (YA4410) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling MAP4K1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MAP4K1 Antibody (YA4410) Product Image
    1/16
  • Experimental Validation Results for MAP4K1 Antibody (YA4410)
  • MAP4K1 Antibody (YA4410) Product Image
  • Experimental Validation Results for MAP4K1 Antibody (YA4410)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling MAP4K1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MAP4K1 Antibody (YA4410)
  • MAP4K1 Antibody (YA4410) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Ramos cells labeling MAP4K1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MAP4K1 Antibody (YA4410) Product Image FC