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Phospho-Histone H2A.X (Ser139) Antibody (YA190)

(Synonyms: H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X)
Customer Review

Based on 1 Customer Validation

Phospho-Histone H2A.X (Ser139) Antibody (YA190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissur Info
 
Dilution Ratio 1:500 1:50-1:500 1:2000 1:500

Product Details

Description

Phospho-Histone H2A.X (Ser139) Antibody (YA190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 15/20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser139 of Human Histone H2AX.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102537

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293(lane 3(20μg) and C6(lane 4(20ug) using Phospho-Histone H2A.X(HY-P80463 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Histone H2A.X is a Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA (Probable). Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140 (PubMed:12419185, PubMed:12607005, PubMed:15201865). These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11, RAD50, and NBN (PubMed:12419185, PubMed:12607005, PubMed:15201865). Interaction with the MRN complex is mediated at least in part by NBN (PubMed:12419185). Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143 (PubMed:15613478). Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (PubMed:16820410). Interacts with WRAP53/TCAB1 (PubMed:26734725, PubMed:27715493). Interacts with HDGFL2 (PubMed:26721387). Interacts with DNA damage up-regulated protein DDUP (PubMed:35849344). Forms a complex with DDUP and RAD18 following DDUP phosphorylation (PubMed:35849344)

  • SwissProt ID

    P16104

  • Gene ID
    3014 [NCBI]
  • Synonyms

    H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

  • Research Field

    Epigenetics and Nuclear Signaling

Phospho-Histone H2A.X (Ser139) Antibody (YA190) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293(lane 3(20μg) and C6(lane 4(20ug) using Phospho-Histone H2A.X(HY-P80463 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
    1/16
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293(lane 3(20μg) and C6(lane 4(20ug) using Phospho-Histone H2A.X(HY-P80463 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Experimental Validation Results for Phospho-Histone H2A.X (Ser139) Antibody (YA190)
  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293(lane 3(20μg) and C6(lane 4(20ug) using Phospho-Histone H2A.X(HY-P80463 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image WB
  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image IHC-P
  • Phospho-Histone H2A.X (Ser139) Antibody (YA190) Product Image IHC-P