ACE Antibody (YA2094)(PBS only)

Cat. No.: HY-P82349A: Data Sheet User Guide for Antibodies
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ACE Antibody (YA2094) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACE.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ACE Antibody (YA2094) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACE.

Host

Rabbit

Molecular Weight

Predicted band size: 151 kDa;

Observed band size: 180 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat

SwissProt ID

P09470

Gene ID

11421 [NCBI]

Immunogen

Recombinant protein of mouse Angiotensin Converting Enzyme 1 aa636 -1237.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Dipeptidyl carboxypeptidase that removes dipeptides from the C-terminus of a variety of circulating hormones, such as angiotensin I, bradykinin or enkephalins, thereby playing a key role in the regulation of blood pressure, electrolyte homeostasis or synaptic plasticity (PubMed:11723129, PubMed:12777443, PubMed:14757757, PubMed:16270063, PubMed:35201898, PubMed:7753170, PubMed:8642790, PubMed:9231832). Composed of two similar catalytic domains, each possessing a functional active site, with different selectivity for substrates (PubMed:11303049). Plays a major role in the angiotensin-renin system that regulates blood pressure and sodium retention by the kidney by converting angiotensin I to angiotensin II, resulting in an increase of the vasoconstrictor activity of angiotensin (PubMed:11303049, PubMed:14757757, PubMed:9231832). Also able to inactivate bradykinin, a potent vasodilator, and therefore enhance the blood pressure response (By similarity). Acts as a regulator of synaptic transmission by mediating cleavage of neuropeptide hormones, such as substance P, neurotensin or enkephalins (By similarity). Catalyzes degradation of different enkephalin neuropeptides (Met-enkephalin, Leu-enkephalin, Met-enkephalin-Arg-Phe and possibly Met-enkephalin-Arg-Gly-Leu) (PubMed:35201898). Acts as a regulator of synaptic plasticity in the nucleus accumbens of the brain by mediating cleavage of Met-enkephalin-Arg-Phe, a strong ligand of Mu-type opioid receptor OPRM1, into Met-enkephalin (PubMed:35201898). Met-enkephalin-Arg-Phe cleavage by ACE decreases activation of OPRM1, leading to long-term synaptic potentiation of glutamate release (PubMed:35201898). Also acts as a regulator of hematopoietic stem cell differentiation by mediating degradation of hemoregulatory peptide N-acetyl-SDKP (AcSDKP) (PubMed:11303049). Acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity). Involved in amyloid-beta metabolism by catalyzing degradation of Amyloid-beta protein 40 and Amyloid-beta protein 42 peptides, thereby preventing plaque formation (By similarity). Catalyzes cleavage of cholecystokinin (maturation of Cholecystokinin-8 and Cholecystokinin-5) and Gonadoliberin-1 (both maturation and degradation) hormones (By similarity). Degradation of hemoregulatory peptide N-acetyl-SDKP (AcSDKP) and amyloid-beta proteins is mediated by the N-terminal catalytic domain, while angiotensin I and cholecystokinin cleavage is mediated by the C-terminal catalytic region (PubMed:11303049); Soluble form that is released in blood plasma and other body fluids following proteolytic cleavage in the juxtamembrane stalk region; Isoform produced by alternative promoter usage that is specifically expressed in spermatocytes and adult testis, and which is required for male fertility (PubMed:1651914, PubMed:9482924). In contrast to somatic isoforms, only contains one catalytic domain (PubMed:16270063). Acts as a dipeptidyl carboxypeptidase that removes dipeptides from the C-terminus of substrates (PubMed:16270063). The identity of substrates that are needed for male fertility is unknown (PubMed:16270063). Isoform Testis-specific and isoform Somatic have distinct activities and cannot completely compensate for the loss of the other when expressed in somatic tissues or testis (PubMed:10831599, PubMed:11723129, PubMed:12777443, PubMed:16270063, PubMed:9664078). May also have a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety (PubMed:15665832). The GPIase activity was reported to be essential for the egg-binding ability of the sperm (PubMed:15665832). This activity is however unclear and has been challenged by other groups, suggesting that it may be indirect (PubMed:16270063)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cytoplasm; Secreted; Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:Highly expressed in the kidneys and lungs; not expressed in the liver (PubMed:16154999) . In the brain, expressed in the cerebral cortex, hippocampus, cerebellum, and basal ganglia/brainstem (PubMed:16154999) . Highly expressed in neurons of the dopamine receptor DRD1 in the dorsal striatum and nucleus accumbens (PubMed:35201898) ; specifically expressed in spermatocytes and adult testes.

Isoforms & Post-Translational Modification：P09470 has 2 isomers: P09470-1: 150918 Da (predicted); P09470-2: 84047 Da (predicted).
Produced following proteolytic cleavage by secretase enzymes that cleave the transmembrane form in the juxtamembrane stalk region upstream of the transmembrane region. Cleavage can take place at different sites of the juxtamembrane stalk region;Phosphorylated by CK2 on Ser-1305; which allows membrane retention (By similarity). Phosphorylated on tyrosine residues on its extracellular part, promoting cleavage by secretase enzymes and formation of the soluble form (Angiotensin-converting enzyme, soluble form) (By similarity)

Subunit：Monomer and homodimer; homodimerizes following binding to an inhibitor (By similarity). Interacts with calmodulin (CALM1, CALM2 or CALM3); interaction takes place in the cytoplasmic region and regulates phosphorylation and proteolytic cleavage (By similarity)

Research Field

Cardiovascular

Synonyms

ACE; Dipeptidyl carboxypeptidase I; Kininase II; Angiotensin Converting Enzyme 1 Antibody

Documentation

User Guide for Antibodies (1077 KB)