SPATA5L1 Antibody (YA7052)

Cat. No.: HY-P87369: Data Sheet User Guide for Antibodies
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SPATA5L1 Antibody (YA7052) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPATA5L1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SPATA5L1 Antibody (YA7052) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPATA5L1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 81 kDa;

Observed band size: 81 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q9BVQ7

Immunogen

Recombinant protein within Human SPATA5L1 aa 379-580 / 753.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed:38554706). Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024)

Subcellular Localization：Cytoplasm,Cytoplasm, cytoskeleton, spindle,Nucleus

Expression：
Tissue_Specificity: Expressed in both neurons and glia during embryonic and adult stages of brain development

Isoforms & Post-Translational Modification：Q9BVQ7 has three isomers: Q9BVQ7-1: 80710 Da (predicted); Q9BVQ7-2: 66111 Da (predicted); Q9BVQ7-3: 41138 Da (predicted).

Subunit：Part of the 55LCC heterohexameric ATPase complex composed at least of AIRIM, AFG2A, AFG2B and CINP (PubMed:35354024, PubMed:38554706)

Synonyms

FLJ12286 antibody; MGC5347 antibody; SPA5L_HUMAN antibody; SPATA5L1 antibody; Spermatogenesis-associated protein 5-like protein 1 antibody

Documentation

User Guide for Antibodies (1077 KB)