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AIM2 Antibody (YA3513)

(Synonyms: PYHIN4)
Customer Review

Based on 1 Customer Validation

AIM2 Antibody (YA3513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AIM2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • AIM2 Antibody (YA3513) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

AIM2 Antibody (YA3513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AIM2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718773

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AIM2 Antibody (YA3513)
    Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AIM2 Antibody (YA3513)
    Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)
    Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    AIM2 is a cytosolic innate immune receptor which recognizes double-stranded DNA (dsDNA) released during cellular perturbation and pathogenic assault.

  • Subcellular Localization

    Cytoplasm; Inflammasome; Nucleus

  • Expression


    Tissue_specificity:Expressed in spleen, small intestine, peripheral blood leukocytes, and testis

    Induction:By IFNG/IFN-gamma and IFNB1/IFN-beta

  • Subunit

    Self-associates; forms homooligomers in response to cytosolic double-stranded DNA (dsDNA) and the dsDNA seems to serve as oligomerization platform (PubMed:15582594, PubMed:19158676, PubMed:24406744, PubMed:26197926, PubMed:26583071, PubMed:29440442, PubMed:33980849).

  • SwissProt ID

    O14862

  • Gene ID
    9447 [NCBI]
  • Synonyms

    PYHIN4

AIM2 Antibody (YA3513) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513) Product Image
    1/16
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • AIM2 Antibody (YA3513) Product Image
  • Experimental Validation Results for AIM2 Antibody (YA3513)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AIM2 Antibody (YA3513)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • Experimental Validation Results for AIM2 Antibody (YA3513)
  • AIM2 Antibody (YA3513) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • AIM2 Antibody (YA3513) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513) Product Image WB
  • AIM2 Antibody (YA3513) Product Image ICC
  • AIM2 Antibody (YA3513) Product Image ICC