NEK7 Antibody (YA1360)
(Synonyms: NEK7; NimA related protein kinase 7)
Based on 1 Customer Validation
NEK7 Antibody (YA1360) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK7.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IP
-
Reactivity :
Mouse, Rat
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IP
IP: Immunoprecipitation
|
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
NEK7 Antibody (YA1360) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK7.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityMouse, Rat Predicted Reactivity: Human
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
-
Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 35 kDa
Entrez Gene: 140609 Human ; 59125 Mouse ; 360850 Rat
SwissProt: Q8TDX7 Human ; Q9ES74 Mouse ; D3ZBE5 Rat
OMIM: 606848 Human
A synthetic peptide of human NEK7 aa17-30.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Western blot analysis of extracts from NIH/3T3(lane 2(20ug), RAW264.7(lane 3(20ug) and Jurkat(lane 4(20ug) using NEK7 Antibody (HY-P81615) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
-
Function
NEK6 is a NIMA-related serine/threonine kinase required for mitotic progression, and loss of NEK6 function arrests cells in M phase and triggers apoptosis[1]. Mechanistically, NEK9/Nercc1 activates NEK6 during mitosis through Ser206 phosphorylation, placing NEK6 in a mitotic kinase cascade important for cell-cycle progression[2]. NEK6 and NEK7 both support spindle formation and cytokinesis, but they are not redundant because NEK6 localizes to spindle microtubules and the midbody more clearly than NEK7[3]. NEK6 also acts on EML4 with NEK7 to reduce microtubule affinity and promote chromosome congression[4]. In cancer models, NEK6 overexpression induces anchorage-independent transformation in JB6 Cl41 cells, whereas NEK6 inhibition promotes premature senescence and cell death in cancer cells[5][6]. For experimental applications, ATP-site NEK6 inhibitors identified by virtual screening provide kinase-focused tools for testing NEK6-dependent tumor-cell phenotypes[7].
-
Subcellular Localization
Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
-
Expression
Tissue_specificity:Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine -
Isoforms & Post-Translational Modification
Q8TDX7 has 2 isomers: Q8TDX7-1: 34551 Da (predicted); Q8TDX7-2: 13402 Da (predicted).
Phosphorylation at Ser-195 required for its activation -
Subunit
Monomer (PubMed:21320329). Interacts with NEK9; interaction takes place during mitosis; it relieves NEK7 autoinhibition and prevents interaction with NLRP3 (PubMed:19414596, PubMed:19941817, PubMed:26522158). Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation (PubMed:26188091). Interacts (via N-terminus) with NLRP3 (via LRR repeat domain); the interaction is required for the formation of the complex NLRP3:PYCARD, oligomerization of PYCARD and activation of CASP1 (PubMed:31189953, PubMed:36442502)
-
SwissProt ID
-
Synonyms
NEK7; NimA related protein kinase 7
-
Research Field
Cell Biology
Documentation
-
Data Sheet (261 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
User Guide for Antibodies (1077 KB)
References
[1]. Yin MJ, et al. The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis. J Biol Chem. 2003 Dec 26;278(52):52454-60. [Content Brief]
[2]. Belham C, et al. A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases. J Biol Chem. 2003 Sep 12;278(37):34897-909. [Content Brief]
[3]. O'Regan L, et al. The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Mol Cell Biol. 2009 Jul;29(14):3975-90. [Content Brief]
[4]. Adib R, et al. Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression. Sci Signal. 2019 Aug 13;12(594):eaaw2939. [Content Brief]
[5]. Jeon YJ, et al. Role of NEK6 in tumor promoter-induced transformation in JB6 C141 mouse skin epidermal cells. J Biol Chem. 2010 Sep 3;285(36):28126-33. [Content Brief]
[6]. Jee HJ, et al. The inhibition of Nek6 function sensitizes human cancer cells to premature senescence upon serum reduction or anticancer drug treatment. Cancer Lett. 2013 Jul 10;335(1):175-82. [Content Brief]
[7]. De Donato M, et al. Identification and antitumor activity of a novel inhibitor of the NIMA-related kinase NEK6. Sci Rep. 2018 Oct 30;8(1):16047. [Content Brief]
Powered by Bioz
