14-3-3 beta Antibody (YA1949)
(Synonyms: YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1)14-3-3 beta Antibody (YA1949) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 14-3-3 beta.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
14-3-3 beta Antibody (YA1949) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 14-3-3 beta.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 27-29 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 28 kDa
Entrez Gene: 7529 Human ; 56011 Rat
SwissProt: P31946 Human ; P63104 Human ; P63101 Mouse ;
OMIM: 601289 Human
A synthetic peptide of human 14-3-3
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
14-3-3 proteins are conserved eukaryotic scaffolding proteins that primarily regulate target-protein function through phosphorylation-dependent binding[1]. Mechanistically, 14-3-3 recognizes phosphoserine-binding motifs and connects protein kinase signaling to protein-complex assembly, localization, and activity control[2][3]. Therefore, 14-3-3 participates in cell-cycle control, apoptosis, signal transduction, energy metabolism, and protein trafficking[1][4]. In disease models, the 14-3-3 interaction network regulates cancer-relevant apoptosis, cell-cycle progression, autophagy, glucose metabolism, and cell motility[5]. Compared with broadly expressed 14-3-3β, γ, ε, ζ, η, and τ, 14-3-3σ shows stronger relevance to cell-cycle and tumor-suppressor biology in cancer research[4][6]. 14-3-3η also acts as a serum biomarker in rheumatoid arthritis by complementing RF and ACPA testing and associating with worse disease[7]. For experimental applications, small-molecule modulators of 14-3-3 proteins support studies of protein interactions, cell cycle, apoptosis, and disease-linked signaling pathways[8].
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Subcellular Localization
Cytoplasm; Melanosome; Vacuole membrane
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Isoforms & Post-Translational Modification
14-3-3 beta has 2 isomers: P31946-1: 28082 Da (predicted); P31946-2: 27850 Da (predicted).
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion -
Subunit
Homodimer (PubMed:17717073). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13 (PubMed:21224381).
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SwissProt ID
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Synonyms
YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
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Research Field
Neuroscience
Documentation
[1]. Obsilova V, et al. Structural insights into the functional roles of 14-3-3 proteins. Front Mol Biosci. 2022;9:1016071. [Content Brief]
[2]. Muslin AJ, et al. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine. Cell. 1996;84(6):889-897. [Content Brief]
[3]. Yang X, et al. Structural basis for protein-protein interactions in the 14-3-3 protein family. Proc Natl Acad Sci U S A. 2006;103(46):17237-17242. [Content Brief]
[4]. Gardino AK, et al. 14-3-3 proteins as signaling integration points for cell cycle control and apoptosis. Semin Cell Dev Biol. 2011;22(7):688-695. [Content Brief]
[5]. Pennington KL, et al. The dynamic and stress-adaptive signaling hub of 14-3-3: emerging mechanisms of regulation and context-dependent protein-protein interactions. Oncogene. 2018;37(42):5587-5604. [Content Brief]
[6]. Li Z, et al. 14-3-3σ, the double-edged sword of human cancers. Am J Transl Res. 2009;1(4):326-340. [Content Brief]
[7]. Maksymowych WP, et al. Serum 14-3-3η is a novel marker that complements current serological measurements to enhance detection of patients with rheumatoid arthritis. J Rheumatol. 2014;41(11):2104-2113. [Content Brief]
[8]. Aghazadeh Y, et al. The role of the 14-3-3 protein family in health, disease, and drug development. Drug Discov Today. 2016;21(2):278-287. [Content Brief]
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